Detailed information of TA system TA177834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3779836..3780362
• Replicon: chromosome
• Accession: NZ_CP063242
• Organism: Pectobacterium carotovorum strain XP-13

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: IHJ55_RS16690
• Protein ID: WP_203470338.1
• Coordinates: 3780057..3780362 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: E0SK21
• Locus tag: IHJ55_RS16685
• Protein ID: WP_012773205.1
• Coordinates: 3779836..3780054 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHJ55_RS16660	3776075..3777334	+	1260	WP_203470334.1	tyrosine-type recombinase/integrase	-
IHJ55_RS16665	3777476..3777646	-	171	WP_162847981.1	hypothetical protein	-
IHJ55_RS16670	3777787..3778128	+	342	WP_203470335.1	hypothetical protein	-
IHJ55_RS16675	3778290..3778463	-	174	WP_203470336.1	hypothetical protein	-
IHJ55_RS16680	3779034..3779777	+	744	WP_203470337.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
IHJ55_RS16685	3779836..3780054	+	219	WP_012773205.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
IHJ55_RS16690	3780057..3780362	+	306	WP_203470338.1	type II toxin-antitoxin system toxin CcdB	Toxin
IHJ55_RS16695	3780516..3782888	-	2373	WP_203470339.1	AAA family ATPase	-
IHJ55_RS16700	3782881..3783600	-	720	WP_203470340.1	hypothetical protein	-
IHJ55_RS16705	3783597..3785030	-	1434	WP_203470341.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3775898..3785743	9845

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11803.69 Da
• Isoelectric Point: 5.6276

>T177834 WP_203470338.1 NZ_CP063242:3780057-3780362 [Pectobacterium carotovorum]
MQFIVYKYKRSSHYRMFVDVQSDIVETPKRRMVIPLIEAHHLSEKVNKTLFPQIRIDGENYRLMTTELSSVPVEVIGEVI
ADLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T177834 NZ_CP083709:c2279692-2279321 [Enterobacter cloacae complex sp. ECL352]
ATGGAGCGCATGGCGGTGTTCGATACCAATATACTCATCGATCTTTTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCTCATCGAGCCATTAGTCTGATCACATGGATGGAAGTCATGGTGGGGGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTTTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAGTTGCCCGTTAATCTCACGCAA
TACGAAAGACTTTTCGGGCATCACCGGGGTCGTTTCACCTTACCAGCTGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8201.16 Da
• Isoelectric Point: 7.2038

>AT177834 WP_012773205.1 NZ_CP063242:3779836-3780054 [Pectobacterium carotovorum]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQHGSFADENRNW

Nucleotide

• Length: 219 bp

>AT177834 NZ_CP083709:c2279937-2279695 [Enterobacter cloacae complex sp. ECL352]
ATGATTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: E0SK21