Detailed information of TA system TA177827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4690226..4691814
• Replicon: chromosome
• Accession: NZ_CP063241
• Organism: Pectobacterium brasiliense strain ZLMLSHJ5

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: IHJ54_RS20795
• Protein ID: WP_203449756.1
• Coordinates: 4690495..4691814 (+)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: IHJ54_RS20790
• Protein ID: WP_103943261.1
• Coordinates: 4690226..4690495 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHJ54_RS20765	4685721..4686272	+	552	WP_119870761.1	TetR/AcrR family transcriptional regulator	-
IHJ54_RS20770	4686303..4686713	-	411	WP_094431545.1	helix-turn-helix transcriptional regulator	-
IHJ54_RS20775	4686856..4687707	+	852	WP_203449753.1	SDR family oxidoreductase	-
IHJ54_RS20780	4687800..4688684	-	885	WP_203449754.1	LysR family transcriptional regulator	-
IHJ54_RS20785	4688897..4690033	+	1137	WP_203449755.1	alpha/beta hydrolase	-
IHJ54_RS20790	4690226..4690495	+	270	WP_103943261.1	helix-turn-helix domain-containing protein	Antitoxin
IHJ54_RS20795	4690495..4691814	+	1320	WP_203449756.1	type II toxin-antitoxin system HipA family toxin	Toxin
IHJ54_RS20800	4692281..4696042	-	3762	WP_203449757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49303.62 Da
• Isoelectric Point: 7.0049

>T177827 WP_203449756.1 NZ_CP063241:4690495-4691814 [Pectobacterium brasiliense]
MAALDVYMNGYRVGILTKTGSGAHHFSYDANWLGLPRSRPISLSMPLRHQPYQGDEVYNFFDNLLPDNPDIRRRIVARHH
TDSTQPFDLLAKVGRDSVGALQLVPQGTPVHDIKKIEYKTLSGQQLENILTGYLSDAPLGMIDTEDDFRISIAGAQEKTA
LLYLDDRWCLPLNATPTTHIIKLPIGKIESHSYSIDMSDSVENEYLCLLIAKAFGLPVPHCFMINAGKIKALAVERFDRK
YASDVSWIMRLPQEDFCQVLNVPSALKYENHGGPGISAIMSFLLGSVDPERDRYRFMQAQVLFWLLAATDGHAKNFSLFI
EPEGRYRLTPFYDILSMYPAMGGRGIDRREAKLAMGLTGSRGKKYAIEQIFPRHFFQTAKVVGFARESMENILAEFAHTV
DTIMATVGSQLPVDFPTHISEAILAGLQARSRRLMSGWE

Nucleotide

• Length: 1320 bp

>T177827 NZ_CP083706:25209-25589 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10062.40 Da
• Isoelectric Point: 9.7281

>AT177827 WP_103943261.1 NZ_CP063241:4690226-4690495 [Pectobacterium brasiliense]
MMKITNSKQLSSYLKDVRITQKLSQGKVASKVGIRQDTVSSFEQHPDSTKLETFFKILSALNLELTVTPRNVDTTNNEAS
VASSWKEEW

Nucleotide

• Length: 270 bp

>AT177827 NZ_CP083706:24988-25209 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA