Detailed information of TA system TA177824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3828987..3829627
• Replicon: chromosome
• Accession: NZ_CP063241
• Organism: Pectobacterium brasiliense strain ZLMLSHJ5

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: IHJ54_RS16930
• Protein ID: WP_203449521.1
• Coordinates: 3828987..3829403 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A086EF97
• Locus tag: IHJ54_RS16935
• Protein ID: WP_039278475.1
• Coordinates: 3829400..3829627 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHJ54_RS16910	3824377..3825363	-	987	WP_203449519.1	quinone oxidoreductase	-
IHJ54_RS16915	3825686..3827092	+	1407	WP_010279223.1	replicative DNA helicase	-
IHJ54_RS16920	3827134..3828216	+	1083	WP_203449520.1	alanine racemase	-
IHJ54_RS16925	3828209..3828847	+	639	WP_039549675.1	hypothetical protein	-
IHJ54_RS16930	3828987..3829403	-	417	WP_203449521.1	type II toxin-antitoxin system VapC family toxin	Toxin
IHJ54_RS16935	3829400..3829627	-	228	WP_039278475.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IHJ54_RS16940	3831086..3831565	+	480	WP_010279234.1	Lrp/AsnC family transcriptional regulator	-
IHJ54_RS16945	3831816..3832430	+	615	WP_014916569.1	YitT family protein	-
IHJ54_RS16950	3832494..3833687	+	1194	WP_039465897.1	aspartate/tyrosine/aromatic aminotransferase	-
IHJ54_RS16955	3833771..3834268	+	498	WP_095700526.1	M48 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15231.77 Da
• Isoelectric Point: 7.7826

>T177824 WP_203449521.1 NZ_CP063241:c3829403-3828987 [Pectobacterium brasiliense]
VSLLFMLDTNICSFIMREHPAEVIEKLQQCTLRRDRIVVSAITYAEMRFGAVGKKASPRHMQLVDAFCRRLDAVLPWDSA
AVDATTKIKIELAAAGTPIGPNDTAIAGHAISAKAILVTNNLREFERVPGLTLEDWVK

Nucleotide

• Length: 417 bp

>T177824 NZ_CP083702:c99856-99551 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8879.08 Da
• Isoelectric Point: 4.8359

>AT177824 WP_039278475.1 NZ_CP063241:c3829627-3829400 [Pectobacterium brasiliense]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEDRADADFMTERQDVVEEGRFKL

Nucleotide

• Length: 228 bp

>AT177824 NZ_CP083702:c100076-99858 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A086EF97