Detailed information of TA system TA177821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1021487..1022001
• Replicon: chromosome
• Accession: NZ_CP063241
• Organism: Pectobacterium brasiliense strain ZLMLSHJ5

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: IHJ54_RS04420
• Protein ID: WP_203450517.1
• Coordinates: 1021487..1021762 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: IHJ54_RS04425
• Protein ID: WP_203450087.1
• Coordinates: 1021783..1022001 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHJ54_RS04400	1016962..1017177	+	216	WP_203450083.1	DUF3577 domain-containing protein	-
IHJ54_RS04405	1017280..1018551	+	1272	WP_203450084.1	hypothetical protein	-
IHJ54_RS04410	1018555..1019091	+	537	WP_203450085.1	hypothetical protein	-
IHJ54_RS04415	1019079..1021019	+	1941	WP_203450086.1	hypothetical protein	-
IHJ54_RS04420	1021487..1021762	-	276	WP_203450517.1	CcdB family protein	Toxin
IHJ54_RS04425	1021783..1022001	-	219	WP_203450087.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
IHJ54_RS04430	1022016..1022264	-	249	WP_010299606.1	helix-turn-helix domain-containing protein	-
IHJ54_RS04435	1022350..1022604	-	255	WP_203450088.1	helix-turn-helix domain-containing protein	-
IHJ54_RS04440	1022708..1022950	+	243	WP_072036092.1	helix-turn-helix domain-containing protein	-
IHJ54_RS04450	1023778..1025931	+	2154	WP_203450089.1	ornithine decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (9-90)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10448.10 Da
• Isoelectric Point: 4.6533

>T177821 WP_203450517.1 NZ_CP063241:c1021762-1021487 [Pectobacterium brasiliense]
IQTNQQVKKQSDIVDTPKRRMVIPLIEAHHLSEKVNKKLFPLIRIDDGEDYRLMTTEISSVPVEVIGEVIADLGEYADEI
KDVINLMFWGI

Nucleotide

• Length: 276 bp

>T177821 NZ_CP083701:4762785-4763120 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGATTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8181.26 Da
• Isoelectric Point: 10.2013

>AT177821 WP_203450087.1 NZ_CP063241:c1022001-1021783 [Pectobacterium brasiliense]
MKRRISIAVDKGDYQILSAAGVNISELVNDALGKEARRIKTEAWKKENREGMENVARFIAKNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT177821 NZ_CP083701:4762537-4762785 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA