Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-DnaT
Location 347530..348061 Replicon chromosome
Accession NZ_CP063241
Organism Pectobacterium brasiliense strain ZLMLSHJ5

Toxin (Protein)


Gene name stbE Uniprot ID A0A086EVU3
Locus tag IHJ54_RS01550 Protein ID WP_039282785.1
Coordinates 347774..348061 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A1V2R9J2
Locus tag IHJ54_RS01545 Protein ID WP_039282788.1
Coordinates 347530..347784 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHJ54_RS01525 342531..344549 - 2019 WP_203449846.1 penicillin-binding protein activator -
IHJ54_RS01530 344611..345498 + 888 WP_040038101.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
IHJ54_RS01540 346014..347330 + 1317 WP_080163527.1 IS4 family transposase -
IHJ54_RS01545 347530..347784 + 255 WP_039282788.1 plasmid stabilization protein Antitoxin
IHJ54_RS01550 347774..348061 + 288 WP_039282785.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IHJ54_RS01555 348240..349025 + 786 WP_010280213.1 4,5-DOPA dioxygenase extradiol -
IHJ54_RS01560 349093..350253 - 1161 WP_203449847.1 glutathionylspermidine synthase family protein -
IHJ54_RS01565 350265..350939 - 675 WP_010280216.1 DUF1190 family protein -
IHJ54_RS01570 351209..352600 - 1392 WP_039522250.1 outer membrane channel protein TolC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 346014..347330 1316


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11011.96 Da        Isoelectric Point: 10.7694

>T177817 WP_039282785.1 NZ_CP063241:347774-348061 [Pectobacterium brasiliense]
MTYKLKFVPSAMKEWKKLGHPVREQFKKKLVERLENPRVPSAQLHGRKDQYKIKLKSAGYRLVYLVQDETITVMVMGVGK
REGSQIYSDTKGRSA

Download         Length: 288 bp

>T177817 NZ_CP083701:4351920-4352234 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9208.38 Da        Isoelectric Point: 3.7947

>AT177817 WP_039282788.1 NZ_CP063241:347530-347784 [Pectobacterium brasiliense]
MAFNILTNTAASITELKRDPMGTVRAAEGETIAILNRNEPAFYCVPPAVFAYLMELAEDAELGRTVDDRMSELADAEEIS
LDDL

Download         Length: 255 bp

>AT177817 NZ_CP083701:4352231-4352647 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A086EVU3

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2R9J2

References