Detailed information of TA system TA177816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 187352..187969
• Replicon: chromosome
• Accession: NZ_CP063241
• Organism: Pectobacterium brasiliense strain ZLMLSHJ5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: IHJ54_RS00810
• Protein ID: WP_039502576.1
• Coordinates: 187790..187969 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A086ESS8
• Locus tag: IHJ54_RS00805
• Protein ID: WP_014916995.1
• Coordinates: 187352..187762 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHJ54_RS00790	183202..184245	-	1044	WP_010281514.1	DNA-binding transcriptional regulator CytR	-
IHJ54_RS00795	184554..186752	-	2199	WP_014916996.1	primosomal protein N'	-
IHJ54_RS00800	187025..187240	+	216	WP_010281510.1	50S ribosomal protein L31	-
IHJ54_RS00805	187352..187762	-	411	WP_014916995.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IHJ54_RS00810	187790..187969	-	180	WP_039502576.1	type II toxin-antitoxin system HicA family toxin	Toxin
IHJ54_RS00815	188053..189063	-	1011	WP_039505047.1	iron ABC transporter permease	-
IHJ54_RS00820	189060..190022	-	963	WP_203449814.1	ABC transporter substrate-binding protein	-
IHJ54_RS00825	190032..190829	-	798	WP_203449815.1	ABC transporter ATP-binding protein	-
IHJ54_RS00830	191064..191381	-	318	WP_039307765.1	met regulon transcriptional regulator MetJ	-
IHJ54_RS00835	191569..192729	+	1161	WP_039499769.1	cystathionine gamma-synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6576.74 Da
• Isoelectric Point: 10.7838

>T177816 WP_039502576.1 NZ_CP063241:c187969-187790 [Pectobacterium brasiliense]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKLGLVTIPHPKKDLPIGTVKSIRKQAGI

Nucleotide

• Length: 180 bp

>T177816 NZ_CP083701:c4303856-4303392 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14506.39 Da
• Isoelectric Point: 4.3391

>AT177816 WP_014916995.1 NZ_CP063241:c187762-187352 [Pectobacterium brasiliense]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLIEMGQDIPTVSSVGQLAKSAEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Nucleotide

• Length: 411 bp

>AT177816 NZ_CP083701:c4304191-4303856 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A086ESS8