Detailed information of TA system TA177794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 295047..295568
• Replicon: plasmid pLP4
• Accession: NZ_CP063236
• Organism: Rhodococcus qingshengii strain 7B

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: T5HL71
• Locus tag: IM699_RS30880
• Protein ID: WP_021334703.1
• Coordinates: 295047..295352 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IM699_RS30885
• Protein ID: WP_030538123.1
• Coordinates: 295353..295568 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM699_RS30860	290413..291471	-	1059	WP_165639875.1	IS110 family transposase	-
IM699_RS30865	291782..292129	+	348	WP_021334700.1	DUF1016 family protein	-
IM699_RS30870	292255..293052	-	798	WP_021334701.1	hypothetical protein	-
IM699_RS30875	293052..294449	-	1398	WP_080656785.1	hypothetical protein	-
IM699_RS30880	295047..295352	-	306	WP_021334703.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IM699_RS30885	295353..295568	-	216	WP_030538123.1	hypothetical protein	Antitoxin
IM699_RS30890	295932..296651	-	720	WP_021334705.1	hypothetical protein	-
IM699_RS30895	296993..297775	+	783	WP_021334706.1	DUF4145 domain-containing protein	-
IM699_RS30900	297913..299227	+	1315	Protein_300	IS256 family transposase	-
IM699_RS30905	299266..299568	+	303	Protein_301	transposase	-
IM699_RS30910	299489..299698	-	210	WP_166503019.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..416450	416450

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-119)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 10515.97 Da
• Isoelectric Point: 4.7318

>T177794 WP_021334703.1 NZ_CP063236:c295352-295047 [Rhodococcus qingshengii]
VRPIHDAQLGAGVSSVLVLTRELVRPHLGTVTVAPIRAEGRGLSTELSLGEGNGVEDGSVVDCDGLTTIQVSALGVNRGF
LLPAQEVLLTRALRSAFDLES

Nucleotide

• Length: 306 bp

>T177794 NZ_CP083690:4025338-4025727 [Serratia sp. HRI]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAAGCGGAACTGCTTTACGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCCGCGGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGCTGCTACGGTGAAATG
CGCGCGAACATGGAGAGAAAGGGCAAAATCATGGGCGCGCTGGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7525.58 Da
• Isoelectric Point: 10.4526

>AT177794 WP_030538123.1 NZ_CP063236:c295568-295353 [Rhodococcus qingshengii]
MSTQIAVRLPEGIVAFLDGEVSGGRAKSRAQVVLRALEREQRRQLAVRDVEILSDGAGGDVNIAAAHGKKS

Nucleotide

• Length: 216 bp

>AT177794 NZ_CP083690:4025071-4025334 [Serratia sp. HRI]
ATGGAAAGAATCGCAAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGGTTACCGGTGGAATTTGAATTTGATACGGA
TCGTGTCTATATCCGTCGCGATCGGGAGGGCAACGTGATTTTATCCAAATATCCGGCAAAGCCGGACAGCTGGGCACCGC
TGCTGAGCATTATCGCCCAAACGCCGGTGCCGCAAGAGTTTCTTGGCGCGGAAGATCGTCAGCAAGGGGTTGCAGACCGC
GATCCATTCTCAGAGGAAGAGTAG

Structures

Toxin

• AlphaFold DB: T5HL71