Detailed information of TA system TA177789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 225414..225961
• Replicon: chromosome
• Accession: NZ_CP063233
• Organism: Pseudomonas fluorescens strain KF1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S6AKK9
• Locus tag: IM720_RS00990
• Protein ID: WP_016502317.1
• Coordinates: 225686..225961 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S6AY38
• Locus tag: IM720_RS00985
• Protein ID: WP_016502318.1
• Coordinates: 225414..225686 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM720_RS00955	220964..222280	+	1317	WP_019334901.1	MFS transporter	-
IM720_RS00960	222316..223059	-	744	WP_019334900.1	conjugal transfer protein TraX	-
IM720_RS00965	223056..223400	-	345	WP_080034398.1	DedA family protein	-
IM720_RS00970	223446..224009	-	564	WP_019334898.1	recombinase family protein	-
IM720_RS00975	224240..224980	+	741	WP_019334897.1	DUF2807 domain-containing protein	-
IM720_RS00980	224987..225400	+	414	WP_016502319.1	hypothetical protein	-
IM720_RS00985	225414..225686	+	273	WP_016502318.1	ribbon-helix-helix domain-containing protein	Antitoxin
IM720_RS00990	225686..225961	+	276	WP_016502317.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IM720_RS00995	226236..227507	+	1272	WP_023082049.1	IS4-like element ISPa1635 family transposase	-
IM720_RS01000	227883..228278	+	396	WP_080034397.1	GtrA family protein	-
IM720_RS01005	228259..229245	+	987	WP_100226690.1	glycosyltransferase family 2 protein	-
IM720_RS01010	229245..230660	+	1416	WP_193689996.1	glycosyltransferase family 39 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	223446..234606	11160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10702.22 Da
• Isoelectric Point: 7.2020

>T177789 WP_016502317.1 NZ_CP063233:225686-225961 [Pseudomonas fluorescens]
MELKWTSKALSDIARLYEFLASVNQPAAARTVQQLTAAPSTLLANPRIGERLEEFDPRDVRRIQIGHYEMRYEIVDSTIY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T177789 NZ_CP083690:c2578838-2578470 [Serratia sp. HRI]
ATGCGAACAATACCCGCGTCATCCACTCGGGCGGCCCCGTCGTGCCCGTCCCCCATGGCTATCTGGCAATTCTTGCTCAC
CTACCTGCTGGCGCAGCACTACGGCCTGACGCTCAACGACACCGCATTCAGCGACGATGCCGTAATACAGGCCCATATCG
GTGACGGTATCTCCCTCGCGGATACGCTGAATGCCATGGTGGAGAAGTTTGATCTGGTACGCATTGACCGCCGAGGGTTC
GACTGTCAGGCGCAATCGCCCTTTATCACTGCTATCGATATTCTCCGCGCCCGTCGAGCCACCGGCCTGATGACCCGTAT
GGGTTACCAGACCATCACCTCAGTTATCCGGGGAGAAAAGCAGGCATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10074.43 Da
• Isoelectric Point: 5.7066

>AT177789 WP_016502318.1 NZ_CP063233:225414-225686 [Pseudomonas fluorescens]
MATTAKTRSVTAHVPVQLAEKVDLMAERLERSKNWIVKQALAAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWAESL
STDTPLPVPR

Nucleotide

• Length: 273 bp

>AT177789 NZ_CP083690:c2579232-2578897 [Serratia sp. HRI]
ATGCCATCAATCATTGCCCCTGTCTGGGGACTTAAACGTAACGTCACTCCGCAGTTTGGCGCCCGCCTGGTACAGGAAGG
CGGGCGACTGCACTTCCTGGCTGACCGTGCCGATATCAGCGGTACCTTCAGCGACGAGCAGCTGCGTGACGTAGATAACG
CCTTCCCGCAGTTCATCAACCACCTGGAACTGATGCTGCTTTCTGGTGAGCTCAATCCCCGTTATCAGCACTGTGTCACG
CTGTATTGCAATGGCTTGACCTGCGAAGCCGACAGTCTGGGATCACAGGGTTATGTGTACCTCTCGATTTATCCGACACC
TGAAACGCCCAGGTAA

Structures

Toxin

• AlphaFold DB: S6AKK9

Antitoxin

• AlphaFold DB: S6AY38