Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hipBA/HipA-HipB |
| Location | 175904..177527 | Replicon | plasmid pBD-50-Eh_2 |
| Accession | NZ_CP063225 | ||
| Organism | Enterobacter hormaechei subsp. steigerwaltii strain BD-50-Eh | ||
Toxin (Protein)
| Gene name | hipA | Uniprot ID | C1IUI3 |
| Locus tag | IE977_RS24005 | Protein ID | WP_000088044.1 |
| Coordinates | 176202..177527 (+) | Length | 442 a.a. |
Antitoxin (Protein)
| Gene name | hipB | Uniprot ID | A0A6M3PBR7 |
| Locus tag | IE977_RS24000 | Protein ID | WP_012477377.1 |
| Coordinates | 175904..176197 (+) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IE977_RS23980 | 171703..172095 | + | 393 | WP_000341066.1 | hypothetical protein | - |
| IE977_RS23985 | 172754..173410 | + | 657 | WP_000462754.1 | trypsin-like serine protease | - |
| IE977_RS23990 | 173613..174110 | + | 498 | WP_000340139.1 | membrane protein | - |
| IE977_RS23995 | 174115..175503 | + | 1389 | WP_000083579.1 | hypothetical protein | - |
| IE977_RS24000 | 175904..176197 | + | 294 | WP_012477377.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| IE977_RS24005 | 176202..177527 | + | 1326 | WP_000088044.1 | type II toxin-antitoxin system HipA family toxin | Toxin |
| IE977_RS24010 | 177588..177794 | + | 207 | WP_000134171.1 | hypothetical protein | - |
| IE977_RS24015 | 177895..178305 | + | 411 | WP_000985909.1 | hypothetical protein | - |
| IE977_RS24020 | 178318..179133 | + | 816 | WP_001641519.1 | HNH endonuclease | - |
| IE977_RS24025 | 179386..179811 | + | 426 | WP_000278471.1 | hypothetical protein | - |
| IE977_RS24030 | 180360..180668 | + | 309 | WP_001224686.1 | hypothetical protein | - |
| IE977_RS24035 | 180684..181541 | + | 858 | WP_000784387.1 | HNH endonuclease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaTEM-1D / dfrA1 / ant(3'')-Ia / qacE / sul1 | - | 1..336282 | 336282 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 442 a.a. Molecular weight: 50333.32 Da Isoelectric Point: 6.5044
>T177767 WP_000088044.1 NZ_CP063225:176202-177527 [Enterobacter hormaechei subsp. steigerwaltii]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
Download Length: 1326 bp
>T177767 NZ_CP083656:241808-242242 [Burkholderia vietnamiensis]
GTGATTACCTACCTTCTCGACGTCAACGTACTCATTGCTTTGCTCGACCCGGCACACGTTCAACACGAGCCGGCGCATGC
GTGGTTTGGCCGGGTAGGCCATTCCGCATGGGCCACGTGCCCGCTCACCGAGAACGGCGTGCTTCGAATTATCGGCAATC
CGAAATACCCGAATACGATGGTCTCTCCTGCATCCGTTGCACCGCTTGTTGTGCAACTGCGGTCCCATGCTGGGCACACA
TTCTGGTCGGACGATGTGAGCCTGCTCGACGAAAAACACGTCGACGCCACCCGTTTACTGTCCTCCGCTCAACTGACCGA
CACTTACCTCCTCGCGCTCGCTCGCGCACACAAAGGCAAGTTGGCGACCTTCGATCGCCGCTTGGTCGTCGACGCGGTTC
CGAACGGCGGGAAGCATCTTGAACTGATTCCTTGA
GTGATTACCTACCTTCTCGACGTCAACGTACTCATTGCTTTGCTCGACCCGGCACACGTTCAACACGAGCCGGCGCATGC
GTGGTTTGGCCGGGTAGGCCATTCCGCATGGGCCACGTGCCCGCTCACCGAGAACGGCGTGCTTCGAATTATCGGCAATC
CGAAATACCCGAATACGATGGTCTCTCCTGCATCCGTTGCACCGCTTGTTGTGCAACTGCGGTCCCATGCTGGGCACACA
TTCTGGTCGGACGATGTGAGCCTGCTCGACGAAAAACACGTCGACGCCACCCGTTTACTGTCCTCCGCTCAACTGACCGA
CACTTACCTCCTCGCGCTCGCTCGCGCACACAAAGGCAAGTTGGCGACCTTCGATCGCCGCTTGGTCGTCGACGCGGTTC
CGAACGGCGGGAAGCATCTTGAACTGATTCCTTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 10860.30 Da Isoelectric Point: 10.3134
>AT177767 WP_012477377.1 NZ_CP063225:175904-176197 [Enterobacter hormaechei subsp. steigerwaltii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW
Download Length: 294 bp
>AT177767 NZ_CP083656:241584-241811 [Burkholderia vietnamiensis]
ATGCGTACCACTATTGATATCGACGACGACGTCCTTGCCTTCGCGCGGGAGCGTGCAAAGCCCGGCACGAGTATCGGGAA
GGTCATTTCTGACCTCGCCCGTGCGGCCCTGCAGCGCGGAACAACGAACCAGTCGAGCCGAAACGGCTTGCCGTTGATGC
CGGTTTCCGCGACCGCCCGTCCGGTCACCCTTGATACCGTTAATCAACTTCGCGACGACTCGCTGTGA
ATGCGTACCACTATTGATATCGACGACGACGTCCTTGCCTTCGCGCGGGAGCGTGCAAAGCCCGGCACGAGTATCGGGAA
GGTCATTTCTGACCTCGCCCGTGCGGCCCTGCAGCGCGGAACAACGAACCAGTCGAGCCGAAACGGCTTGCCGTTGATGC
CGGTTTCCGCGACCGCCCGTCCGGTCACCCTTGATACCGTTAATCAACTTCGCGACGACTCGCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2S1XXW1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6M3PBR7 |