Detailed information of TA system TA177766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4732015..4732631
• Replicon: chromosome
• Accession: NZ_CP063224
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain BD-50-Eh

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IE977_RS22670
• Protein ID: WP_015569913.1
• Coordinates: 4732015..4732386 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: IE977_RS22675
• Protein ID: WP_015569912.1
• Coordinates: 4732389..4732631 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE977_RS22655	4729515..4730417	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
IE977_RS22660	4730414..4731049	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
IE977_RS22665	4731046..4731975	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
IE977_RS22670	4732015..4732386	-	372	WP_015569913.1	PIN domain-containing protein	Toxin
IE977_RS22675	4732389..4732631	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
IE977_RS22680	4732830..4733750	+	921	WP_047718170.1	alpha/beta hydrolase	-
IE977_RS22685	4733759..4734700	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
IE977_RS22690	4734745..4735182	-	438	WP_045348649.1	D-tyrosyl-tRNA(Tyr) deacylase	-
IE977_RS22695	4735179..4736060	-	882	WP_003861949.1	virulence factor BrkB family protein	-
IE977_RS22700	4736054..4736653	-	600	WP_003861947.1	glucose-1-phosphatase	-
IE977_RS22705	4736772..4737572	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13767.90 Da
• Isoelectric Point: 6.4882

>T177766 WP_015569913.1 NZ_CP063224:c4732386-4732015 [Enterobacter hormaechei subsp. steigerwaltii]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVSARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T177766 NZ_CP083655:2731522-2731923 [Burkholderia vietnamiensis]
ATGCCGCGCTACATGCTCGACACCAACATGTGCATCTACTTGATGAAGAGCCAGCCCGAGCAAGTGGCGAAGCGATTTGC
GCAGTGCTACACGGGCGACGTCGTGATGTCGGCGATCACGTGTGCCGAGCTGGAGTACGGCGTCACCGCTTGCGCGGCGC
CCGCGCGTGAGCGCCGCCATCTTGCGGCGCTGATCGAGGATATTCCCGTCGCGCCGTTCGACGCCGCGGCCGCGCAAGCA
TATGGGCCCGTGCGTGACGCAACGCGCGCATGGAAGAAGGATCATCTCGACAAACTGATCGCCGCTCATGCGGTGTCGCT
CGATGTCGTGCTGGTGACGAATGACGAGCGCGATTTCACGAGCTATCCGGGATTGCGGCTGGAGAACTGGCTGAACGATT
AG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT177766 WP_015569912.1 NZ_CP063224:c4732631-4732389 [Enterobacter hormaechei subsp. steigerwaltii]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT177766 NZ_CP083655:2731286-2731522 [Burkholderia vietnamiensis]
ATGCATACCACGAGGGTATTTCGGAACGGCAATTCGCAGGCCGTGCGAATTCCTGCGGATCTCGCTTATGAGCGCAGCGA
TATCGAGCTCGAGATCGAGCGCATCGGCGACGAGATTCGCATCCGGCCGGCGCGACGGCCGTTGACGCAGGTGCTCGAGA
AGTTCGCGAAGTTCGCGCCCGATTTCATGGCGGAAGGGCGTGGCGACCACGAGCAGGCCGAACGCGAGGGGTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3