Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 174004..175627 Replicon plasmid pHKU57_1
Accession NZ_CP063216
Organism Klebsiella pneumoniae strain HKU57

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag IM695_RS26045 Protein ID WP_000088044.1
Coordinates 174004..175329 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag IM695_RS26050 Protein ID WP_012477377.1
Coordinates 175334..175627 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM695_RS26015 170157..170717 - 561 WP_001161490.1 recombinase family protein -
IM695_RS26020 170970..171374 + 405 Protein_176 helix-turn-helix domain-containing protein -
IM695_RS26025 171426..172130 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
IM695_RS26030 172398..173213 - 816 WP_001641519.1 HNH endonuclease -
IM695_RS26035 173226..173636 - 411 WP_000985909.1 hypothetical protein -
IM695_RS26040 173737..173943 - 207 WP_000134171.1 hypothetical protein -
IM695_RS26045 174004..175329 - 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
IM695_RS26050 175334..175627 - 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
IM695_RS26055 175850..176852 - 1003 Protein_183 IS110 family transposase -
IM695_RS26060 176931..177365 - 435 WP_003131969.1 mercury resistance transcriptional regulator MerR -
IM695_RS26065 177437..177787 + 351 WP_003131974.1 mercuric ion transporter MerT -
IM695_RS26070 177800..178075 + 276 WP_003131987.1 mercury resistance system periplasmic binding protein MerP -
IM695_RS26075 178147..179832 + 1686 WP_003156770.1 mercury(II) reductase -
IM695_RS26080 179850..180215 + 366 WP_000995360.1 mercury resistance co-regulator MerD -
IM695_RS26085 180212..180499 + 288 WP_050484830.1 broad-spectrum mercury transporter MerE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / dfrA25 / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / armA / blaDHA-1 / qnrB4 - 1..273789 273789
- inside IScluster/Tn aph(6)-Id / aph(3'')-Ib - 167187..182180 14993


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T177752 WP_000088044.1 NZ_CP063216:c175329-174004 [Klebsiella pneumoniae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T177752 NZ_CP083651:c1925809-1925706 [Citrobacter amalonaticus]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT177752 WP_012477377.1 NZ_CP063216:c175627-175334 [Klebsiella pneumoniae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT177752 NZ_CP083651:1925700-1925849 [Citrobacter amalonaticus]
ACAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTCTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References