Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/ElaA-DUF1778
Location 160704..161455 Replicon plasmid pHKU57_1
Accession NZ_CP063216
Organism Klebsiella pneumoniae strain HKU57

Toxin (Protein)


Gene name ataT Uniprot ID H6U1U8
Locus tag IM695_RS25975 Protein ID WP_014386536.1
Coordinates 160704..161186 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A071LPN3
Locus tag IM695_RS25980 Protein ID WP_004902250.1
Coordinates 161177..161455 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM695_RS25965 155706..158963 + 3258 WP_194085737.1 DEAD/DEAH box helicase family protein -
IM695_RS25970 158968..160236 + 1269 WP_155687819.1 ISL3 family transposase -
IM695_RS25975 160704..161186 - 483 WP_014386536.1 GNAT family N-acetyltransferase Toxin
IM695_RS25980 161177..161455 - 279 WP_004902250.1 DUF1778 domain-containing protein Antitoxin
IM695_RS25985 161574..161786 - 213 WP_004902255.1 hypothetical protein -
IM695_RS25990 161894..162235 - 342 WP_004902257.1 hypothetical protein -
IM695_RS25995 163065..163523 - 459 WP_014386535.1 hypothetical protein -
IM695_RS26000 164735..165619 + 885 WP_004186900.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / dfrA25 / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / armA / blaDHA-1 / qnrB4 - 1..273789 273789
- flank IS/Tn - - 158968..160236 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-144)

Antitoxin

Vibrio phage ICP1, Orf50

(10-86)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17662.45 Da        Isoelectric Point: 8.9130

>T177751 WP_014386536.1 NZ_CP063216:c161186-160704 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Download         Length: 483 bp

>T177751 NZ_CP083651:c480529-480311 [Citrobacter amalonaticus]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCTTGCGACGCTGTCAGACAATTGACACACTTGAACGCGT
GATTGAGAAAAATAAATATGAATTATCTGACAATGAATTGGCTGTATTTTACTCAGCCGCCGATCACCGTCTTGCAGAAT
TGACGATGAATAAACTTTACGACAAGATCCCCTCCTCCGTCTGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10704.23 Da        Isoelectric Point: 4.5271

>AT177751 WP_004902250.1 NZ_CP063216:c161455-161177 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Download         Length: 279 bp

>AT177751 NZ_CP083651:c480931-480557 [Citrobacter amalonaticus]
ATGGATGAATACTCACCCAAAAGACATGATATAGCGCAGCTTAAATTTCTTTGTGAAACGCTTTATCACGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTTCAACTCAACGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGAGCAGATTGATGAATATCTG
GACGACACATTTATGTTGTTCAGCAGTTATGGCATTAATGCGCAGGATCTGCAGAAATGGCGGAAATCAGGTAATCGCTT
GTTCCGTTGCTTTGTGAATGCCACCAGAGCTAACCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MBI1

Antitoxin

Source ID Structure
AlphaFold DB A0A071LPN3

References