Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3688721..3689218 Replicon chromosome
Accession NZ_CP063214
Organism Escherichia coli strain ERP001

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IL987_RS17820 Protein ID WP_001173343.1
Coordinates 3689045..3689218 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag IL987_RS17815 Protein ID WP_001312331.1
Coordinates 3688721..3689017 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL987_RS17795 3685450..3686169 - 720 WP_000611291.1 two-component system response regulator DcuR -
IL987_RS17800 3686166..3687797 - 1632 WP_001216482.1 sensor histidine kinase -
IL987_RS17805 3687978..3688208 + 231 WP_000373293.1 (4Fe-4S)-binding protein -
IL987_RS17810 3688220..3688492 + 273 WP_000405647.1 N-acetyltransferase -
IL987_RS17815 3688721..3689017 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IL987_RS17820 3689045..3689218 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IL987_RS17825 3689337..3690854 - 1518 WP_000003806.1 lysine--tRNA ligase -
IL987_RS17830 3691091..3692548 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3688721..3719721 31000


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T177720 WP_001173343.1 NZ_CP063214:3689045-3689218 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T177720 NZ_CP083638:3865910-3866017 [Escherichia fergusonii]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT177720 WP_001312331.1 NZ_CP063214:3688721-3689017 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT177720 NZ_CP083638:c3865852-3865798 [Escherichia fergusonii]
CAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References