Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2202019..2202673 Replicon chromosome
Accession NZ_CP063214
Organism Escherichia coli strain ERP001

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag IL987_RS10720 Protein ID WP_000244765.1
Coordinates 2202019..2202426 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IL987_RS10725 Protein ID WP_000354046.1
Coordinates 2202407..2202673 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL987_RS10700 2197976..2199709 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
IL987_RS10705 2199715..2200425 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IL987_RS10710 2200450..2201346 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IL987_RS10715 2201458..2201979 + 522 WP_001055874.1 flavodoxin FldB -
IL987_RS10720 2202019..2202426 - 408 WP_000244765.1 protein YgfX Toxin
IL987_RS10725 2202407..2202673 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IL987_RS10730 2202916..2203896 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
IL987_RS10735 2203973..2204632 - 660 WP_000250274.1 hemolysin III family protein -
IL987_RS10740 2204796..2205107 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
IL987_RS10745 2205152..2206585 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T177716 WP_000244765.1 NZ_CP063214:c2202426-2202019 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T177716 NZ_CP083638:2869193-2869345 [Escherichia fergusonii]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT177716 WP_000354046.1 NZ_CP063214:c2202673-2202407 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT177716 NZ_CP083638:c2869144-2869087 [Escherichia fergusonii]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References