Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pasABC/RelE-HTH
Location 4991032..4991512 Replicon chromosome
Accession NZ_CP063196
Organism Thermobifida halotolerans strain DSM 44931

Toxin (Protein)


Gene name pasB Uniprot ID A0A399FYU0
Locus tag NI17_RS22300 Protein ID WP_068690293.1
Coordinates 4991032..4991304 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name pasA Uniprot ID A0A399G1K2
Locus tag NI17_RS22305 Protein ID WP_068690295.1
Coordinates 4991288..4991512 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NI17_RS22280 (NI17_022155) 4986627..4988690 + 2064 WP_279395509.1 RNA degradosome polyphosphate kinase -
NI17_RS22285 (NI17_022160) 4988683..4989672 + 990 WP_068690415.1 NUDIX domain-containing protein -
NI17_RS22290 (NI17_022165) 4989731..4990543 + 813 WP_068690290.1 RNA methyltransferase -
NI17_RS22295 (NI17_022170) 4990592..4991044 + 453 WP_068690292.1 SRPBCC family protein -
NI17_RS22300 (NI17_022175) 4991032..4991304 - 273 WP_068690293.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NI17_RS22305 (NI17_022180) 4991288..4991512 - 225 WP_068690295.1 DUF6290 family protein Antitoxin
NI17_RS22310 (NI17_022185) 4991609..4992670 - 1062 WP_068690297.1 ABC transporter ATP-binding protein -
NI17_RS22315 (NI17_022190) 4992667..4993506 - 840 WP_068690299.1 ABC transporter permease -
NI17_RS22320 (NI17_022195) 4993484..4994248 - 765 WP_068690301.1 molybdate ABC transporter substrate-binding protein -
NI17_RS22325 (NI17_022200) 4994417..4995757 + 1341 WP_234401823.1 sensor histidine kinase -
NI17_RS22330 (NI17_022205) 4995760..4996419 + 660 WP_068690303.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-88)

Antitoxin

Relaxosome protein TraY

(4-49)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10689.40 Da        Isoelectric Point: 11.4446

>T177687 WP_068690293.1 NZ_CP063196:c4991304-4991032 [Thermobifida halotolerans]
MTWTIEFDEVARRDLGKLDRQEARRVLRFLNERIAPAEDPRVLGDALKGARLGGLWRYRVGDNRIIVEIHDRLIRVLVVR
IGHRREIYRS

Download         Length: 273 bp

>T177687 NZ_CP083630:c1115894-1115583 [Leclercia adecarboxylata]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGTAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGCTGTA
CCGCTTAGTCCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCGAACTCTGATACCGAGCCTGGACAATTTTAAGTACC
GTAATAAGTGGTGGGTGTTGGATATAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTGAATAAACGCTTC
TATGTGAAGCACATCGCGACCCACGCTGATTACGATAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8484.52 Da        Isoelectric Point: 4.1957

>AT177687 WP_068690295.1 NZ_CP063196:c4991512-4991288 [Thermobifida halotolerans]
MLALRLPEELEQRLAALAQETGRSKSFYAREAIAAYIDDLEDVYLAERRIEDLRAGRSHTIPLSEVMAEYDLDD

Download         Length: 225 bp

>AT177687 NZ_CP083630:c1115586-1115167 [Leclercia adecarboxylata]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCTGTCCCCTTCCTGGGGGGCAGTTCGTC
CGAGAGCGATTACCGCGAAGCGATGGAGTTGGTGGACTATCTGATCGCGAACGACGACGAGAACCCGCTTATTGACTTCC
TGGCGAGTAAAATTGCCGACTATGAGGATAACAGCCCTCGTTTCGCTGAATTCAACAAGGCTGTCGCTGAAATGCCGGTC
GGCGTTGCCCTTCTGCGCACCCTGATTGACCAGTACAAGCTGTCTTATTCTGACCTGAAGGAGGAGATCGGCTCTAAATC
GCTGGTTAGCCAGATTCTCTCTGGCCAGCGGTCACTGACAATCACGCACATTAAGGCGCTGTCTGCTCGTTTTGGTGTTA
AACCAGAGTGGTTCTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A399FYU0

Antitoxin

Source ID Structure
AlphaFold DB A0A399G1K2

References