Detailed information of TA system TA177676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 675912..677380
• Replicon: plasmid unnamed
• Accession: NZ_CP063167
• Organism: Variovorax paradoxus strain VAI-C

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: INQ48_RS34130
• Protein ID: WP_203207909.1
• Coordinates: 675912..676778 (-)
• Length: 289 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: INQ48_RS34135
• Protein ID: WP_203207910.1
• Coordinates: 676775..677380 (-)
• Length: 202 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INQ48_RS34105	671723..672868	+	1146	Protein_573	alpha-hydroxy-acid oxidizing protein	-
INQ48_RS34110	672985..673953	+	969	WP_203207905.1	tripartite tricarboxylate transporter substrate binding protein	-
INQ48_RS34115	674009..674902	+	894	WP_203207906.1	TauD/TfdA family dioxygenase	-
INQ48_RS34120	674916..675626	+	711	WP_203207907.1	4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase	-
INQ48_RS34125	675667..675852	-	186	WP_203207908.1	hypothetical protein	-
INQ48_RS34130	675912..676778	-	867	WP_203207909.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
INQ48_RS34135	676775..677380	-	606	WP_203207910.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
INQ48_RS34140	677664..679127	-	1464	WP_203207911.1	tannase/feruloyl esterase family alpha/beta hydrolase	-
INQ48_RS34145	679297..680925	-	1629	WP_203207912.1	thiamine pyrophosphate-binding protein	-
INQ48_RS34150	680944..682179	-	1236	WP_203207913.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA / adeG / kdsA	1..2479635	2479635

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-256)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (8-55)

Sequences

Toxin

Protein

• Length: 289 a.a.
• Molecular weight: 31660.61 Da
• Isoelectric Point: 8.0562

>T177676 WP_203207909.1 NZ_CP063167:c676778-675912 [Variovorax paradoxus]
VSKDLSASVRARLLNIAKAQGVDFNQVLVRFALERMLYRLGESVHADRFVLKGALLFTLWYDMPHRATRDADLLGFGASD
LASMAQAFRDIASVRVDDGISFDPASIRVSEIRKDAGYAGARVLIDADVARARCKTQIDVGFGDAITPGPADAIYPVLIA
DLPAPRLRTYPAYTVVSEKLHAIVVLGMTNSRLKDYLDLMVLLEREDLDDDMLANAIAATFMRRGMAVPRSLPVGLSDEY
ANDPSRQALWHAFLKKNDMPSRPLVDVVVLLRGRLGPDMRKAGSLHAP

Nucleotide

• Length: 867 bp

>T177676 NZ_CP083620:3576888-3577106 [Enterobacter bugandensis]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTTGAACGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGCCTTGCAGAGC
TAACGATGAATAAGCTGTATGACAAAATCCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 202 a.a.
• Molecular weight: 22514.15 Da
• Isoelectric Point: 9.8728

>AT177676 WP_203207910.1 NZ_CP063167:c677380-676775 [Variovorax paradoxus]
VRQDTHAQRILDLLRRKGMLRASDLDATNTPRVALTRMTDAGLLEKVGRGLYRLPEPGGSEHESLAAIATKVPQAVFCLL
TALQFHELTTQLPRQVWIAMPRGSHVPRIAYPPLKMVQVSGEAYSAGIEVVERDQVPLRVYGVAKTVVDCFKHRNKIGLD
VALEALRDARAQRKASADELWHYARICRVANVMRPYLEAIE

Nucleotide

• Length: 606 bp

>AT177676 NZ_CP083620:3576486-3576860 [Enterobacter bugandensis]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGCAACCATGGCTGGGTAAATGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACACTCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGTCTTTCCTGTTAA