Detailed information of TA system TA177671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1699358..1699956
• Replicon: chromosome
• Accession: NZ_CP063162
• Organism: Geobacillus stearothermophilus strain DG-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IMZ17_RS08615
• Protein ID: WP_193443973.1
• Coordinates: 1699627..1699956 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A7U9J7G7
• Locus tag: IMZ17_RS08610
• Protein ID: WP_023634676.1
• Coordinates: 1699358..1699627 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMZ17_RS08570	1695352..1695651	+	300	WP_014195759.1	transporter suffix domain-containing protein	-
IMZ17_RS08575	1695661..1696458	+	798	WP_193443968.1	SGNH/GDSL hydrolase family protein	-
IMZ17_RS08580	1696455..1697369	+	915	WP_193443969.1	ABC transporter ATP-binding protein	-
IMZ17_RS08585	1697362..1698342	+	981	WP_193443971.1	ABC transporter permease	-
IMZ17_RS08590	1698410..1698658	-	249	Protein_1637	hypothetical protein	-
IMZ17_RS08610	1699358..1699627	+	270	WP_023634676.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IMZ17_RS08615	1699627..1699956	+	330	WP_193443973.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IMZ17_RS08620	1700178..1701011	+	834	WP_193443975.1	pirin family protein	-
IMZ17_RS08625	1701108..1701857	+	750	WP_044744326.1	oxygen-insensitive NADPH nitroreductase	-
IMZ17_RS08630	1701931..1702674	+	744	WP_063329651.1	NAD-dependent protein deacylase	-
IMZ17_RS08635	1702854..1704032	+	1179	WP_063329650.1	acyl-CoA dehydrogenase family protein	-
IMZ17_RS08640	1704069..1704833	+	765	WP_047818345.1	3-oxoacyl-ACP reductase FabG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-107)

Antitoxin

• SpoVT-AbrB domain (17-61)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11900.66 Da
• Isoelectric Point: 7.1324

>T177671 WP_193443973.1 NZ_CP063162:1699627-1699956 [Geobacillus stearothermophilus]
MQAPDRGDLVYVNFNPQAGHEQAGKGPGIVLSPKRFNQLTGFAVLCPITRQQKGYPFEVGLPSGLAVEGVILTDQVKSLD
WRARQLQIVGRAPNEVVSDCLDLIHTFLS

Nucleotide

• Length: 330 bp

>T177671 NZ_CP083620:88744-89055 [Enterobacter bugandensis]
ATGCTATTCATTGAAACGGACATTTTCACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATCGGTTCCAGAT
ATTCCTGGCGACACAACCGGAGTACGGCGATGTTATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GAAAGGGAAAGCGTGGTGGAGTCAGAGTTATCTATTTTTACCGTACGTGTGAGTTTGAAATCAGACTGCTGCTCATCTAT
CGCAAAGGGATTAAAGATGACCTGTCTGCGGGGGAGAAAGCTATTCTGAAAAAGATGATAGAGAGGTGGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9960.61 Da
• Isoelectric Point: 7.2050

>AT177671 WP_023634676.1 NZ_CP063162:1699358-1699627 [Geobacillus stearothermophilus]
MELNKQKGVSIMTITVQKWGNSLAVRIPSVIAERLALHQGSEVEVIVENQAIKLIPKKKKPTLEELLAKITPENRHAEID
FGTEGNELF

Nucleotide

• Length: 270 bp

>AT177671 NZ_CP083620:89056-89346 [Enterobacter bugandensis]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCGTTGGGCAAATGAACGAGATTGCCGAGGGCCAACGCGAGCCGTC
GCGTATCTTTCAAATCGATGCGATGAAAATAAAAGAAATTCGTCAGGCATCCGGGTTGTCGCAGAGTAAATTCGCGACCT
TAATCTCTGTCAGCGTTGATACATTGCGTAACTGGGAACAAGGCAGACGCTCGCCTACAGGCCCGGCTAAAGCGCTTTTG
CGCGCTATTGCTAACGATCCTCAACATGTCATCCCTGCGCTATCGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9J7G7