Detailed information of TA system TA177670

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 987401..988217
• Replicon: chromosome
• Accession: NZ_CP063162
• Organism: Geobacillus stearothermophilus strain DG-1

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: G8MYR9
• Locus tag: IMZ17_RS05090
• Protein ID: WP_014195290.1
• Coordinates: 987401..987820 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: IMZ17_RS05095
• Protein ID: WP_023634220.1
• Coordinates: 987810..988217 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMZ17_RS05065	984176..984694	+	519	WP_193443604.1	GNAT family N-acetyltransferase	-
IMZ17_RS05070	984829..986034	+	1206	WP_193443606.1	DUF3533 domain-containing protein	-
IMZ17_RS05075	986059..986271	+	213	WP_020278664.1	ATP-binding cassette domain-containing protein	-
IMZ17_RS05080	986238..986708	+	471	Protein_938	ABC transporter permease	-
IMZ17_RS05085	986720..987373	+	654	WP_193443608.1	TetR/AcrR family transcriptional regulator	-
IMZ17_RS05090	987401..987820	-	420	WP_014195290.1	DUF86 domain-containing protein	Toxin
IMZ17_RS05095	987810..988217	-	408	WP_023634220.1	nucleotidyltransferase domain-containing protein	Antitoxin
IMZ17_RS05100	988498..990630	-	2133	WP_193443609.1	ATP-dependent Clp protease ATP-binding subunit	-
IMZ17_RS05105	990898..991644	+	747	WP_063330222.1	FixH family protein	-
IMZ17_RS05110	991814..992854	+	1041	WP_081212641.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (14-131)

Antitoxin

• Polymerase beta, nucleotidyltransferase (9-98)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15907.43 Da
• Isoelectric Point: 8.2859

>T177670 WP_014195290.1 NZ_CP063162:c987820-987401 [Geobacillus stearothermophilus]
MKSDVILNKISVIERCLKRIREEYNGDPKNLQNYTKQDSIVLNLQRACEACIDLAMHIVAEQKFGLPQHSRDAFALLEEH
GVISPSISKKMKAMVGFRNIAVHDYQQLNLGILQAIVEHHLDDFKQFTKAILDYAKKNS

Nucleotide

• Length: 420 bp

>T177670 NZ_CP083613:c4083618-4083208 [Enterobacter hormaechei subsp. oharae]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15269.50 Da
• Isoelectric Point: 5.2680

>AT177670 WP_023634220.1 NZ_CP063162:c988217-987810 [Geobacillus stearothermophilus]
MPNEMETIIIQTLRPALHPFVIYLFGSAARGAMRPDSDVDIAFVSDGEPHDPYELFRLAGHLADKLGRDVDLVDLRQAST
VFQAQVVSTGKAIDCRDERKRAEFEMKTLKMYVKLNEERAPVLKQITESGSIYEK

Nucleotide

• Length: 408 bp

>AT177670 NZ_CP083613:c4083865-4083599 [Enterobacter hormaechei subsp. oharae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: G8MYR9