Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 223217..223853 Replicon chromosome
Accession NZ_CP063162
Organism Geobacillus stearothermophilus strain DG-1

Toxin (Protein)


Gene name mazF Uniprot ID L7ZVP1
Locus tag IMZ17_RS01225 Protein ID WP_003253417.1
Coordinates 223503..223853 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A087LE06
Locus tag IMZ17_RS01220 Protein ID WP_033010302.1
Coordinates 223217..223498 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMZ17_RS01200 219469..220086 - 618 WP_193443192.1 rhomboid family intramembrane serine protease -
IMZ17_RS01205 220205..220594 + 390 WP_193443194.1 holo-ACP synthase -
IMZ17_RS01210 220674..221693 + 1020 WP_193443196.1 outer membrane lipoprotein carrier protein LolA -
IMZ17_RS01215 221936..223102 + 1167 WP_033010307.1 alanine racemase -
IMZ17_RS01220 223217..223498 + 282 WP_033010302.1 hypothetical protein Antitoxin
IMZ17_RS01225 223503..223853 + 351 WP_003253417.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IMZ17_RS01230 224396..226558 + 2163 WP_193443198.1 RNA-binding transcriptional accessory protein -
IMZ17_RS01235 226616..226729 - 114 WP_094239478.1 cortex morphogenetic protein CmpA -
IMZ17_RS01240 226822..227283 + 462 WP_033016555.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12992.00 Da        Isoelectric Point: 4.8781

>T177669 WP_003253417.1 NZ_CP063162:223503-223853 [Geobacillus stearothermophilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T177669 NZ_CP083613:c3198146-3197793 [Enterobacter hormaechei subsp. oharae]
GTGTGGGCGATTAAAACTACGGACAGGTTTGACGACTGGTTCACCTCATTAAACGATAGCGAGAGAGCCAGCGTACTGGC
CGCGCTACTTGTATTGCGGGAAAAGGGACCGGGTCTGTCCAGACCGTATGCCGATACGCTGAAAGGCTCCCGCCACAGCA
ACATGAAAGAATTGCGTATCCAGAGTAAGGGTGATCCGCTGAGGGCATTTTTCGCTTTCAATCCGAACCGTACGGGTATT
GTACTGTGTGCGGGCAATAAGATCGGTAATGAAAGGCGATTTTACGATGAGATGCTCCTCGTTGCGGATCGGGAATACAC
TCGCTGGCTGAACACATTAAAAGAGAGGAATTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10683.20 Da        Isoelectric Point: 7.2027

>AT177669 WP_033010302.1 NZ_CP063162:223217-223498 [Geobacillus stearothermophilus]
VSESGATTEIIVRLPQSLLTELDVLVKQENGNRNELIYQATKMYIRERKKRQIREAMRRGYMEMAKINLSIASEAFHAEY
EADHTVERLVSGG

Download         Length: 282 bp

>AT177669 NZ_CP083613:c3197788-3197486 [Enterobacter hormaechei subsp. oharae]
ATGGGCAGAACCCTGGAGCAGCTTATTGCAGATGAAAAACCTGAGGTTGTTGCCAGCGCACAGGCAATGGCAACGGATAT
TTTGCTCAATATCCATCTGGCCGAGTTACGTGAAAAAGTGCAAAAAACGCAGGTAGATATGGCCAGGGCGTTGGGTATTA
AACAACCTACTGTCGCCGTCATGGAAAAGGCAGGGCGCGATATTAAGCTTTCTTCTTTGAAACGCTACGTTGAGGCCGCC
GGAGGGAAGCTACGGCTGGATGTAGAACTGCCCGATGGTTCTCATTACGAGTTTGTGTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9ERQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A087LE06

References