Detailed information of TA system TA177668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2338351..2339329
• Replicon: chromosome
• Accession: NZ_CP063158
• Organism: Bacillus cereus strain CF4-51

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: IMY50_RS12165
• Protein ID: WP_016081623.1
• Coordinates: 2338351..2339088 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: IMY50_RS12170
• Protein ID: WP_000237818.1
• Coordinates: 2339201..2339329 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMY50_RS12140 (IMY50_12135)	2333966..2334373	+	408	WP_000063589.1	VOC family protein	-
IMY50_RS12145 (IMY50_12140)	2334386..2334775	+	390	Protein_2304	SAM-dependent methyltransferase	-
IMY50_RS12150 (IMY50_12145)	2334933..2336618	-	1686	WP_081367270.1	alpha-keto acid decarboxylase family protein	-
IMY50_RS12155 (IMY50_12150)	2336726..2337208	+	483	WP_071714838.1	MarR family winged helix-turn-helix transcriptional regulator	-
IMY50_RS12160 (IMY50_12155)	2337375..2338112	+	738	WP_071714839.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
IMY50_RS12165 (IMY50_12160)	2338351..2339088	+	738	WP_016081623.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
IMY50_RS12170 (IMY50_12165)	2339201..2339329	+	129	WP_000237818.1	hypothetical protein	Antitoxin
IMY50_RS12175 (IMY50_12170)	2339402..2339578	+	177	WP_000852629.1	stage II sporulation protein SB	-
IMY50_RS12180 (IMY50_12175)	2339598..2339987	-	390	WP_000713869.1	YxeA family protein	-
IMY50_RS12185 (IMY50_12180)	2340199..2341668	+	1470	WP_071714840.1	beta-Ala-His dipeptidase	-
IMY50_RS12190 (IMY50_12185)	2341914..2342723	+	810	WP_000664543.1	papain-like cysteine protease family protein	-
IMY50_RS12195 (IMY50_12190)	2342749..2343351	+	603	WP_071714841.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28351.09 Da
• Isoelectric Point: 8.2998

>T177668 WP_016081623.1 NZ_CP063158:2338351-2339088 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFTDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPSVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIIIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T177668 NZ_CP083613:c3046207-3046104 [Enterobacter hormaechei subsp. oharae]
GGCAGGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT177668 WP_000237818.1 NZ_CP063158:2339201-2339329 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT177668 NZ_CP083613:3046099-3046300 [Enterobacter hormaechei subsp. oharae]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTCAATCGCCCTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGCAAAAGTGGT
CTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Structures

Antitoxin

• AlphaFold DB: W8YZL5