177667

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 244757..245399 Replicon chromosome
Accession NZ_CP063158
Organism Bacillus cereus strain CF4-51

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag IMY50_RS01415 Protein ID WP_000635965.1
Coordinates 245049..245399 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag IMY50_RS01410 Protein ID WP_000004570.1
Coordinates 244757..245044 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMY50_RS01385 (IMY50_01385) 240075..241037 + 963 WP_071713816.1 UV DNA damage repair endonuclease UvsE -
IMY50_RS01390 (IMY50_01390) 241030..241601 - 572 Protein_225 rhomboid family intramembrane serine protease -
IMY50_RS01395 (IMY50_01395) 241695..242054 + 360 WP_071713817.1 holo-ACP synthase -
IMY50_RS01400 (IMY50_01400) 242211..243161 + 951 WP_002094231.1 outer membrane lipoprotein carrier protein LolA -
IMY50_RS01405 (IMY50_01405) 243279..244448 + 1170 WP_071713818.1 alanine racemase -
IMY50_RS01410 (IMY50_01410) 244757..245044 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
IMY50_RS01415 (IMY50_01415) 245049..245399 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IMY50_RS01420 (IMY50_01420) 245468..247636 + 2169 WP_071713819.1 Tex family protein -
IMY50_RS01425 (IMY50_01425) 247694..247810 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
IMY50_RS01430 (IMY50_01430) 248005..248463 + 459 WP_071713820.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T177667 WP_000635965.1 NZ_CP063158:245049-245399 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T177667 NZ_CP083613:c2640096-2639611 [Enterobacter hormaechei subsp. oharae]
GTGGGACGTGTAACAGCACCAGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTAAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGGAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATTATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT177667 WP_000004570.1 NZ_CP063158:244757-245044 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT177667 NZ_CP083613:c2640350-2640084 [Enterobacter hormaechei subsp. oharae]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACAGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTATGCCGAGTTCATTGACATGCTCGATGCCCCGGTCGCGAACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References