Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | spoIISABC/SpoIISA-SpoIISB |
| Location | 2632651..2633568 | Replicon | chromosome |
| Accession | NZ_CP063157 | ||
| Organism | Bacillus velezensis strain GY65 | ||
Toxin (Protein)
| Gene name | spoIISA | Uniprot ID | I2HQ15 |
| Locus tag | IMZ24_RS12605 | Protein ID | WP_007407256.1 |
| Coordinates | 2632651..2633397 (+) | Length | 249 a.a. |
Antitoxin (Protein)
| Gene name | spoIISB | Uniprot ID | I2HQ14 |
| Locus tag | IMZ24_RS12610 | Protein ID | WP_003154807.1 |
| Coordinates | 2633398..2633568 (+) | Length | 57 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IMZ24_RS12585 | 2628044..2628994 | - | 951 | WP_032874618.1 | ring-cleaving dioxygenase | - |
| IMZ24_RS12590 | 2629316..2630632 | + | 1317 | WP_032874616.1 | amino acid permease | - |
| IMZ24_RS12595 | 2630918..2631535 | + | 618 | WP_032874614.1 | DUF47 domain-containing protein | - |
| IMZ24_RS12600 | 2631548..2632546 | + | 999 | WP_032874611.1 | inorganic phosphate transporter | - |
| IMZ24_RS12605 | 2632651..2633397 | + | 747 | WP_007407256.1 | type II toxin-antitoxin system SpoIISA family toxin | Toxin |
| IMZ24_RS12610 | 2633398..2633568 | + | 171 | WP_003154807.1 | type II toxin-antitoxin system SpoIISB family antitoxin | Antitoxin |
| IMZ24_RS12615 | 2633825..2634703 | - | 879 | WP_032874609.1 | N-acetylmuramoyl-L-alanine amidase | - |
| IMZ24_RS12620 | 2634717..2634980 | - | 264 | WP_003154813.1 | phage holin | - |
| IMZ24_RS12625 | 2634994..2635257 | - | 264 | WP_003154815.1 | hemolysin XhlA family protein | - |
| IMZ24_RS12630 | 2635309..2636070 | - | 762 | WP_032874607.1 | hypothetical protein | - |
| IMZ24_RS12635 | 2636127..2636324 | - | 198 | WP_032874605.1 | XkdX family protein | - |
| IMZ24_RS12640 | 2636329..2636700 | - | 372 | WP_032874603.1 | YomQ/XkdW protein, phage-like element PBSX | - |
| IMZ24_RS12645 | 2636713..2638335 | - | 1623 | WP_032874601.1 | pyocin knob domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 2606458..2680506 | 74048 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 249 a.a. Molecular weight: 29077.57 Da Isoelectric Point: 4.7755
>T177665 WP_007407256.1 NZ_CP063157:2632651-2633397 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG
Download Length: 747 bp
>T177665 NZ_CP083613:c1909991-1909518 [Enterobacter hormaechei subsp. oharae]
ATGATTTTTTATCGTCTGGTAACAGGACGTTTTGCCAGCGAAGCGTGGAGTGGAAGTGGTGCAAACCAGTATGGAGGACG
CTGGAATCATAAAGGCCATCCTGCCGTGTATGTCTCCACCTCCATCTCGCTGGCTTCACTGGAAATTCTGGTGCACGTCA
GAAAAGACACCGTGCTGAACCAGTACCAGCTTTTCAGCATTAATATCCCGGATGATCAGATTGATTACCTGGATAAAGCG
TGGCTGCCCGAGGACTGGCAGGAAAATCCGGCGCCGGTATCAACGATGGATCTGGGTACCGGTTGGTTGCAGGCTAACAG
CGCTCTCGCACTCATCTTACCCTCCTGCATCATTCCCTATGAAAACAACGCCATACTTAATCCATTGCATCCGGCATTCC
GCAAAGCACTCAATTCTGTTGAATTACTGCCTTTTGATTTTGATACCCGTCTCGCAGAAAAAACCGCACCCTGA
ATGATTTTTTATCGTCTGGTAACAGGACGTTTTGCCAGCGAAGCGTGGAGTGGAAGTGGTGCAAACCAGTATGGAGGACG
CTGGAATCATAAAGGCCATCCTGCCGTGTATGTCTCCACCTCCATCTCGCTGGCTTCACTGGAAATTCTGGTGCACGTCA
GAAAAGACACCGTGCTGAACCAGTACCAGCTTTTCAGCATTAATATCCCGGATGATCAGATTGATTACCTGGATAAAGCG
TGGCTGCCCGAGGACTGGCAGGAAAATCCGGCGCCGGTATCAACGATGGATCTGGGTACCGGTTGGTTGCAGGCTAACAG
CGCTCTCGCACTCATCTTACCCTCCTGCATCATTCCCTATGAAAACAACGCCATACTTAATCCATTGCATCCGGCATTCC
GCAAAGCACTCAATTCTGTTGAATTACTGCCTTTTGATTTTGATACCCGTCTCGCAGAAAAAACCGCACCCTGA
Antitoxin
Download Length: 57 a.a. Molecular weight: 6676.74 Da Isoelectric Point: 10.9273
>AT177665 WP_003154807.1 NZ_CP063157:2633398-2633568 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA
Download Length: 171 bp
>AT177665 NZ_CP083613:c1910431-1909988 [Enterobacter hormaechei subsp. oharae]
ATGAGAACATGGGTTCCTGCGCAAAGGTCGGCTGATAACACGCTGTGGCGCTACGCCGGTTTACCTGCAAGCAGAGGCAT
GCGGTTGATTGAATATCTCAATCAAGGATTACCCGTCAGCGTCCTCGACAATATTCACGAGTGGACGGACATGTCGAAAG
CGGAGATTTTACGCGTCACGGGAATTAACGAGCGTAACGTTGCCCGCCGCAAAAGTACGGGCCGGACGCTGACGCCCGAC
GAGAGCGAACGTGTGGCGCGTTTCGTTCGGGTACTGGATGCGGCCGTCGATTATTTCGGCAGTAAAGAAGAGGCATGGAA
CTGGTTGCAGTTACCGGTACGTGGGCTTGGCAATGTTGCACCTGTCGATCTGATTGCCACGGAAACCGGTGCGCTGGAAG
TTACCGATTTGATTGGTCGGCTGGAGCACGGAGTGTTCGCATGA
ATGAGAACATGGGTTCCTGCGCAAAGGTCGGCTGATAACACGCTGTGGCGCTACGCCGGTTTACCTGCAAGCAGAGGCAT
GCGGTTGATTGAATATCTCAATCAAGGATTACCCGTCAGCGTCCTCGACAATATTCACGAGTGGACGGACATGTCGAAAG
CGGAGATTTTACGCGTCACGGGAATTAACGAGCGTAACGTTGCCCGCCGCAAAAGTACGGGCCGGACGCTGACGCCCGAC
GAGAGCGAACGTGTGGCGCGTTTCGTTCGGGTACTGGATGCGGCCGTCGATTATTTCGGCAGTAAAGAAGAGGCATGGAA
CTGGTTGCAGTTACCGGTACGTGGGCTTGGCAATGTTGCACCTGTCGATCTGATTGCCACGGAAACCGGTGCGCTGGAAG
TTACCGATTTGATTGGTCGGCTGGAGCACGGAGTGTTCGCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|