Detailed information of TA system TA177664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 8365..8866
• Replicon: plasmid unnamed3
• Accession: NZ_CP063156
• Organism: Escherichia coli strain M7424

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A2S5RIK4
• Locus tag: INR48_RS24515
• Protein ID: WP_022645141.1
• Coordinates: 8365..8625 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: A0A2S5RIE4
• Locus tag: INR48_RS24520
• Protein ID: WP_022645142.1
• Coordinates: 8615..8866 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INR48_RS24475	3965..4126	+	162	WP_097297158.1	type I toxin-antitoxin system Hok family toxin	-
INR48_RS24480	4276..4872	+	597	WP_000125164.1	hypothetical protein	-
INR48_RS24485	4890..5174	-	285	WP_001162371.1	hypothetical protein	-
INR48_RS24490	5248..5913	-	666	WP_000532148.1	P-loop NTPase	-
INR48_RS24495	6510..6839	+	330	WP_000855121.1	hypothetical protein	-
INR48_RS24500	7418..7741	+	324	WP_000427402.1	hypothetical protein	-
INR48_RS24505	7782..7991	+	210	WP_000866037.1	hypothetical protein	-
INR48_RS24510	8089..8313	+	225	WP_000814383.1	hypothetical protein	-
INR48_RS24515	8365..8625	+	261	WP_022645141.1	hypothetical protein	Toxin
INR48_RS24520	8615..8866	+	252	WP_022645142.1	transcriptional regulator	Antitoxin
INR48_RS24525	9416..9694	+	279	WP_000545938.1	hypothetical protein	-
INR48_RS24530	10081..10263	-	183	WP_001401700.1	hypothetical protein	-
INR48_RS24535	10821..11003	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
INR48_RS24540	11028..11465	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
INR48_RS24545	11596..11916	-	321	WP_001310435.1	hypothetical protein	-
INR48_RS24550	12250..12428	-	179	Protein_17	hypothetical protein	-
INR48_RS24555	12430..12882	-	453	WP_000206702.1	hypothetical protein	-
INR48_RS24560	12902..13501	-	600	WP_000091423.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..61773	61773
	-	flank	IS/Tn	-	-	2298..3446	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9876.23 Da
• Isoelectric Point: 7.2030

>T177664 WP_022645141.1 NZ_CP063156:8365-8625 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMS
EEKNAN

Nucleotide

• Length: 261 bp

>T177664 NZ_CP083613:c1400051-1399833 [Enterobacter hormaechei subsp. oharae]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
AATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTTTGGAAATTTGTTCGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9407.81 Da
• Isoelectric Point: 9.8120

>AT177664 WP_022645142.1 NZ_CP063156:8615-8866 [Escherichia coli]
MLTENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT177664 NZ_CP083613:c1400453-1400079 [Enterobacter hormaechei subsp. oharae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTCAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A2S5RIK4

Antitoxin

• AlphaFold DB: A0A2S5RIE4