Detailed information of TA system TA177663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 73502..74103
• Replicon: plasmid unnamed2
• Accession: NZ_CP063155
• Organism: Escherichia coli strain M7424

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A1M0S1Q8
• Locus tag: INR48_RS24370
• Protein ID: WP_024236459.1
• Coordinates: 73723..74103 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: INR48_RS24365
• Protein ID: WP_001190712.1
• Coordinates: 73502..73723 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INR48_RS24320	69381..69614	+	234	WP_000516537.1	hypothetical protein	-
INR48_RS24325	69793..70086	+	294	WP_193475637.1	hypothetical protein	-
INR48_RS24330	70093..70467	+	375	WP_000988654.1	hypothetical protein	-
INR48_RS24335	70449..71129	+	681	WP_193475638.1	hypothetical protein	-
INR48_RS24340	71113..71397	+	285	WP_001142392.1	hypothetical protein	-
INR48_RS24345	71397..71702	+	306	WP_000988220.1	hypothetical protein	-
INR48_RS24350	71699..72223	+	525	WP_000159954.1	toprim domain-containing protein	-
INR48_RS24355	72284..72865	-	582	WP_089648953.1	DNA-binding protein	-
INR48_RS24360	73040..73429	+	390	WP_000506727.1	DNA repair protein	-
INR48_RS24365	73502..73723	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
INR48_RS24370	73723..74103	+	381	WP_024236459.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
INR48_RS24375	74108..74287	+	180	WP_000113018.1	hypothetical protein	-
INR48_RS24380	74315..75358	+	1044	WP_097337022.1	DUF968 domain-containing protein	-
INR48_RS24385	75447..75899	+	453	WP_001326849.1	late promoter-activating protein	-
INR48_RS24390	75985..77178	+	1194	WP_089648951.1	terminase	-
INR48_RS24395	77178..78662	+	1485	WP_000124159.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96139	96139

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13643.37 Da
• Isoelectric Point: 5.1514

>T177663 WP_024236459.1 NZ_CP063155:73723-74103 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T177663 NZ_CP083613:1267641-1268009 [Enterobacter hormaechei subsp. oharae]
ATGAAAACTTTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCTCCAGTGGCTGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATCAAACCGTTATACAGGAACATATCG
ATGCGGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAAAAGTATGAGTTAGTACGTATCGATCGCACAGGATTT
ACCTCACAGGAACAAGCGCCATATTTGACCGTGACAGATATCCTTCATGCCAGGAGAGCATGCGGATTAATGAACAGTTG
TTCGTATCGGGAAGTAAGTAATATTGTTCTTAGCCGATCGCGGCAGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT177663 WP_001190712.1 NZ_CP063155:73502-73723 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT177663 NZ_CP083613:1267300-1267620 [Enterobacter hormaechei subsp. oharae]
ATGAGGAGTAACGCTACATGGGGACTGCAACGTGATATCACACCGCGTCTGGGAGCCCGTCTGGTGCAGGAGGGCAACCG
GCTCCACTATCTGGCTGACCGGGCAAGTATCACCGGTAAATTCAGTGACGCCGAATGCCTGAAGTTGGATGTCGCATTCC
CGCATTTTATCAGTCTGATGGAATCAATGCTGACTACTGGTGAAATGAACCCTCGCCATGCCCACTGTGTCACCCTGTAC
CGCAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGCTGCGGCTACTTATATATCGTTGTTTATCCCACTCAAAGCTA
A

Structures

Toxin

• AlphaFold DB: A0A1M0S1Q8

Antitoxin

• AlphaFold DB: A0A829CJB6