Detailed information of TA system TA177662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 72173..72798
• Replicon: plasmid unnamed1
• Accession: NZ_CP063154
• Organism: Escherichia coli strain M7424

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: INR48_RS23585
• Protein ID: WP_000911315.1
• Coordinates: 72173..72571 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: INR48_RS23590
• Protein ID: WP_000450532.1
• Coordinates: 72571..72798 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INR48_RS23570	68454..68975	+	522	WP_000632670.1	conjugal transfer entry exclusion protein TraS	-
INR48_RS23575	69000..69731	+	732	WP_000850422.1	complement resistance protein TraT	-
INR48_RS23580	69984..72164	+	2181	WP_193475627.1	type IV conjugative transfer system coupling protein TraD	-
INR48_RS23585	72173..72571	-	399	WP_000911315.1	tRNA(fMet)-specific endonuclease VapC	Toxin
INR48_RS23590	72571..72798	-	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / iroE	1..130357	130357

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14871.17 Da
• Isoelectric Point: 8.5264

>T177662 WP_000911315.1 NZ_CP063154:c72571-72173 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQARPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T177662 NZ_CP083613:c636614-636312 [Enterobacter hormaechei subsp. oharae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT177662 WP_000450532.1 NZ_CP063154:c72798-72571 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT177662 NZ_CP083613:c636305-636018 [Enterobacter hormaechei subsp. oharae]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAAACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5