Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 22419..22944 Replicon plasmid unnamed1
Accession NZ_CP063154
Organism Escherichia coli strain M7424

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag INR48_RS23270 Protein ID WP_001159871.1
Coordinates 22639..22944 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag INR48_RS23265 Protein ID WP_000813639.1
Coordinates 22419..22637 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INR48_RS23245 18520..18867 - 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -
INR48_RS23250 18864..19244 - 381 WP_001171554.1 IS66 family insertion sequence hypothetical protein -
INR48_RS23255 19335..20750 - 1416 Protein_15 NTPase -
INR48_RS23260 20752..21840 - 1089 WP_000952217.1 transcriptional repressor PifC -
INR48_RS23265 22419..22637 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
INR48_RS23270 22639..22944 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
INR48_RS23275 22997..23729 + 733 Protein_19 phage integrase family protein -
INR48_RS23280 24516..25271 + 756 WP_000852143.1 replication initiation protein RepE -
INR48_RS23285 25859..27025 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / iroE 1..130357 130357


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T177661 WP_001159871.1 NZ_CP063154:22639-22944 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T177661 NZ_CP083613:c636305-636018 [Enterobacter hormaechei subsp. oharae]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAAACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT177661 WP_000813639.1 NZ_CP063154:22419-22637 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT177661 NZ_CP083613:c636614-636312 [Enterobacter hormaechei subsp. oharae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References