Detailed information of TA system TA177657

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3795815..3796650
• Replicon: chromosome
• Accession: NZ_CP063153
• Organism: Escherichia coli strain M7424

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: INR48_RS18095
• Protein ID: WP_000854726.1
• Coordinates: 3796273..3796650 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: INR48_RS18090
• Protein ID: WP_001377046.1
• Coordinates: 3795815..3796183 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INR48_RS18065	3793246..3793479	+	234	WP_001117564.1	DUF905 family protein	-
INR48_RS18070	3793570..3794388	+	819	WP_001234746.1	DUF945 domain-containing protein	-
INR48_RS18075	3794480..3794965	+	486	WP_000206667.1	antirestriction protein	-
INR48_RS18080	3794981..3795457	+	477	WP_001186773.1	RadC family protein	-
INR48_RS18085	3795520..3795741	+	222	WP_000692346.1	DUF987 domain-containing protein	-
INR48_RS18090	3795815..3796183	+	369	WP_001377046.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
INR48_RS18095	3796273..3796650	+	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
INR48_RS18100	3796647..3797135	+	489	WP_000761657.1	hypothetical protein	-
INR48_RS18105	3797147..3797344	+	198	WP_000839267.1	DUF957 domain-containing protein	-
INR48_RS18110	3797429..3798273	+	845	Protein_3530	DUF4942 domain-containing protein	-
INR48_RS18115	3798344..3799879	+	1536	WP_000492888.1	EAL domain-containing protein	-
INR48_RS18120	3800276..3800446	+	171	Protein_3532	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T177657 WP_000854726.1 NZ_CP063153:3796273-3796650 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T177657 NZ_CP083613:369847-370197 [Enterobacter hormaechei subsp. oharae]
ATGTGGACGGTACTGTTTAGTAAGGTATTCGAGCAATGGTTGCTTGAACAGGAAGATGGGTTGCAGGAAAAGGTATTGGC
AGACCTGTTAAATCTACAGAATTATGGACCGCGTCTTCCTCGTCCCTATGCGGATACCGTTAAAGGGTCACAGTATAAGA
ATATGAAAGAGTTACGTATTCAATATGCGGGTCGCCCGGTTCGTGCGTTCTTCGCGTTTGATCCTGTACGTCAGGCCATT
GTGCTTTGCGCGGGCGATAAGCGTAATGACAAAACCTTTTATGAAAAACTGATTCGCATTGCAGATGCCGAATTCACCCT
TCATCTGACAAGCCTGGAGGCAGCCAAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13584.37 Da
• Isoelectric Point: 6.6255

>AT177657 WP_001377046.1 NZ_CP063153:3795815-3796183 [Escherichia coli]
VSDTLSGTTHPDDNDDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGVTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT177657 NZ_CP083613:370194-370493 [Enterobacter hormaechei subsp. oharae]
GTGAAAACCTTAAATGAAGTGATTGCGTCACGCTCACCGGAAAGCCAACAGCGCATTCAGGAGATGGCAGAGGAAATGTT
CCTCGAAACCGGTCTGCAACTTCTGCGTGAAGAGCTCCAGCTTTCGCAAAAACATGTCGCCGATCTTATGGGCGTAAGTC
AACCTGCGGTAACACAGCTGGAGCAGCGTGGAAACGATCTTAAGATTGCTACGCTTAAGCGTTACATCGAAGCGATGGGC
GGAAAACTTAGCATTAACGTGGAACTTCCCACTGGCAAGCGGGTTGCGTTTCATCTCTAG

Structures

Toxin

• AlphaFold DB: S1EZ92