Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3706556..3707210 Replicon chromosome
Accession NZ_CP063153
Organism Escherichia coli strain M7424

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag INR48_RS17620 Protein ID WP_000244777.1
Coordinates 3706556..3706963 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag INR48_RS17625 Protein ID WP_000354046.1
Coordinates 3706944..3707210 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INR48_RS17600 3702513..3704246 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
INR48_RS17605 3704252..3704962 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
INR48_RS17610 3704987..3705883 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
INR48_RS17615 3705995..3706516 + 522 WP_001055874.1 flavodoxin FldB -
INR48_RS17620 3706556..3706963 - 408 WP_000244777.1 toxin CptA Toxin
INR48_RS17625 3706944..3707210 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
INR48_RS17630 3707453..3708433 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
INR48_RS17635 3708629..3709288 - 660 WP_000250274.1 hemolysin III family protein -
INR48_RS17640 3709452..3709763 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
INR48_RS17645 3709808..3711241 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
INR48_RS17650 3711298..3712041 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T177656 WP_000244777.1 NZ_CP063153:c3706963-3706556 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T177656 NZ_CP083613:366354-366674 [Enterobacter hormaechei subsp. oharae]
ATGAATACTCAACCTGCAACAACTCCGCAGGCGGCGAAGCCATGTCTGTCGCCCCTGGCTGTCTGGAAAATGTTACTGAC
AATCCTGCTAGACCAGCACTATGGTCTCACGCTAAACGACACGCCGTTCAGTGATGAAACTGTTATTCAGAAACACATTG
AGGCAGGTATTACCTTGGCTGATGCCATAAATTTTCTGGTTGAACGCTATGAACTAGTTCGTATTGACCAAAAAGGCTTC
TCTGTGCAAGATCAAGAACCTTGGTTAACTTCTATGGATGTCCACCGAGCACGATTTAAACTCAACGTGGCACGTTTATA
A

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT177656 WP_000354046.1 NZ_CP063153:c3707210-3706944 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT177656 NZ_CP083613:366007-366333 [Enterobacter hormaechei subsp. oharae]
ATGTCATTAATACCAGCCCATGAATGGGGACTACAAAGAGAAATTGTTCCACGGTTTGGTGCCAGATTAGTTCAGGAAGG
CAACCGGCTGCATTATCTGGCTGACAGGGCTAGTTTGATGGGCAATTTCAGCGATACGGAATGTTTCAAATTGAATGACG
CATTTCCACACTTTATCAGCCAGATGGAATCGATGCTGACCACTGGTGAACTGATCCCCTGTCACCATCACTGTGTCACT
CTCTACCACAACGGTTTTACCTGCGAAGCTGACACCCTTGGCAGTTGCGGCTACGTGTACATCGCTGTTTACCCCACTCA
ACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References