Detailed information of TA system TA177639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1518438..1519143
• Replicon: chromosome
• Accession: NZ_CP063153
• Organism: Escherichia coli strain M7424

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: INR48_RS07100
• Protein ID: WP_000539521.1
• Coordinates: 1518757..1519143 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: INR48_RS07095
• Protein ID: WP_001280945.1
• Coordinates: 1518438..1518767 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INR48_RS07080	1513601..1514512	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
INR48_RS07085	1514690..1517032	+	2343	WP_001376787.1	EAL domain-containing protein	-
INR48_RS07090	1517040..1518368	+	1329	WP_000086879.1	GGDEF domain-containing protein	-
INR48_RS07095	1518438..1518767	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
INR48_RS07100	1518757..1519143	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
INR48_RS07105	1519369..1520694	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
INR48_RS07110	1520907..1521290	+	384	WP_000497137.1	biofilm formation regulator BssR	-
INR48_RS07115	1521401..1522516	+	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
INR48_RS07120	1522513..1523139	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T177639 WP_000539521.1 NZ_CP063153:c1519143-1518757 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T177639 NZ_CP083610:2456139-2456474 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT177639 WP_001280945.1 NZ_CP063153:c1518767-1518438 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT177639 NZ_CP083610:2455891-2456139 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6