Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 509101..509737 Replicon chromosome
Accession NZ_CP063150
Organism Bacillus subtilis strain s-16

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag IMY14_RS02600 Protein ID WP_003156187.1
Coordinates 509387..509737 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag IMY14_RS02595 Protein ID WP_003225183.1
Coordinates 509101..509382 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMY14_RS02575 (505460) 505460..506059 - 600 WP_015482831.1 rhomboid family intramembrane serine protease -
IMY14_RS02580 (506154) 506154..506519 + 366 WP_015252768.1 holo-ACP synthase -
IMY14_RS02585 (506685) 506685..507701 + 1017 WP_080030712.1 outer membrane lipoprotein carrier protein LolA -
IMY14_RS02590 (507816) 507816..508985 + 1170 WP_015252766.1 alanine racemase -
IMY14_RS02595 (509101) 509101..509382 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
IMY14_RS02600 (509387) 509387..509737 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IMY14_RS02605 (509853) 509853..510677 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
IMY14_RS02610 (510682) 510682..511047 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
IMY14_RS02615 (511051) 511051..511452 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
IMY14_RS02620 (511464) 511464..512471 + 1008 WP_047182124.1 phosphoserine phosphatase RsbU -
IMY14_RS02625 (512533) 512533..512862 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
IMY14_RS02630 (512859) 512859..513341 + 483 WP_003234299.1 anti-sigma B factor RsbW -
IMY14_RS02635 (513307) 513307..514095 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
IMY14_RS02640 (514095) 514095..514694 + 600 WP_015382836.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T177619 WP_003156187.1 NZ_CP063150:509387-509737 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T177619 NZ_CP083602:c1929563-1929213 [Macrococcus armenti]
ATGAAACGTGGAGATGTTTATTTAGCTGATTTGTCACCGGTAAAGGGATCAGAACAAGGCGGTAAACGCCCTGTTGTCAT
TATTCAGAACAATATAGGTAATAAATATAGCCCAACAGTAATTGTCGCGGCAATTACAGCAAAGATTAACAAAGCAAAGA
TTCCCACCCACGTTGAAATCGGAAAGACGAGTCATCACCTGGAAAAGGATTCGGTAATTCTGTTGGAACAAATAAGAACG
ATAGATAAGAATAGATTGATTGATCATTTAACTGTATTAGATAAAGTGAAGATGAAAGAAGTGGATGTTGCGCTTGCAAT
TAGTCTGGGATTATCAATAGAGAGTGAATAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT177619 WP_003225183.1 NZ_CP063150:509101-509382 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT177619 NZ_CP083602:c1929704-1929564 [Macrococcus armenti]
ATGAGCCAGAAGCTGGAACAAATGCTTATTGAAGGTTATCAATCTATGGCAGAGATTAATTTATCGATTGCATCAGAGCA
TTACATCTTAGAATGTGAAGTAAACGATAACCATCAGACATTAGCACTAGATGGTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References