Detailed information of TA system TA177618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 193432..194189
• Replicon: plasmid pKP19-3088-375k
• Accession: NZ_CP063149
• Organism: Klebsiella pneumoniae strain KP19-3088

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J4ZXV7
• Locus tag: IMY63_RS27965
• Protein ID: WP_029497408.1
• Coordinates: 193707..194189 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: IMY63_RS27960
• Protein ID: WP_094645258.1
• Coordinates: 193432..193719 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMY63_RS27945	189605..190974	+	1370	WP_094645257.1	IS3 family transposase	-
IMY63_RS27950	191500..192072	+	573	WP_094645259.1	cytochrome b/b6 domain-containing protein	-
IMY63_RS27955	192272..193195	+	924	WP_000167917.1	cation diffusion facilitator family transporter	-
IMY63_RS27960	193432..193719	+	288	WP_094645258.1	DUF1778 domain-containing protein	Antitoxin
IMY63_RS27965	193707..194189	+	483	WP_029497408.1	GNAT family N-acetyltransferase	Toxin
IMY63_RS27970	194528..194689	+	162	WP_017900895.1	type I toxin-antitoxin system Hok family toxin	-
IMY63_RS27975	194897..195157	-	261	WP_009653224.1	hypothetical protein	-
IMY63_RS27980	195697..196668	+	972	WP_009653249.1	hypothetical protein	-
IMY63_RS27985	197031..197408	-	378	WP_042927811.1	hypothetical protein	-
IMY63_RS27990	198943..199158	+	216	WP_009653220.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / aac(3)-IId / mph(A) / sul1 / blaNDM-1 / qacE / qnrB6 / aadA16 / dfrA27 / ARR-3	katA	1..375474	375474
	-	flank	IS/Tn	-	-	190171..190974	803

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17599.34 Da
• Isoelectric Point: 9.8719

>T177618 WP_029497408.1 NZ_CP063149:193707-194189 [Klebsiella pneumoniae]
VGRLTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLRLP

Nucleotide

• Length: 483 bp

>T177618 NZ_CP083602:c1117813-1117541 [Macrococcus armenti]
ATGCCTAAGAAAAAGATACTATTTTATTCAGGTGGTTTTGAAGATTATACGTATTGGCAAACTAACGATAAAACTGTATT
AAAGAAAATAAAAAAACTAATAAAGAGTATTATGATTGATGGTGCAAATGAAGGTATAGGTAAGCCAGAAAAATTATTAG
GAAACTATACAGGTTGCTATAGCAGAAGGATCACTCATGAACATAGGCTCGTATATAAGATAGAAGATGATTCAATATGT
ATTTTACAATGTCGTTTTCATTATAAAAGGTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10955.66 Da
• Isoelectric Point: 4.5141

>AT177618 WP_094645258.1 NZ_CP063149:193432-193719 [Klebsiella pneumoniae]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVVDD
PVIEKLLARKPQWDV

Nucleotide

• Length: 288 bp

>AT177618 NZ_CP083602:c1118063-1117806 [Macrococcus armenti]
ATGAAAGTCACAACTTATTCTAATGCAAGATCAAATTTTAGAAAAATAATAGATATTGTTAATGAAGACTGTGAACCTTA
CACAATCACAACAAATACGCATAATGCCGTGATATTATCTGAACAAGATTATAATAGTATGATTGAAACACTTTACTTAC
AGAGTACACCAACGAATGCCGAAAGATTACAAAAAGCGATTGATGAATCAATTGATAAAAATAACTTACTTCAAGTTGAG
ATTGATTTAGATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A2J4ZXV7