Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5251603..5252228 Replicon chromosome
Accession NZ_CP063147
Organism Klebsiella pneumoniae strain KP19-3088

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IMY63_RS25750 Protein ID WP_002882817.1
Coordinates 5251603..5251986 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IMY63_RS25755 Protein ID WP_004150355.1
Coordinates 5251986..5252228 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMY63_RS25735 5248969..5249871 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IMY63_RS25740 5249868..5250503 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IMY63_RS25745 5250500..5251429 + 930 WP_193525095.1 formate dehydrogenase accessory protein FdhE -
IMY63_RS25750 5251603..5251986 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IMY63_RS25755 5251986..5252228 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IMY63_RS25760 5252433..5253350 + 918 WP_002882812.1 alpha/beta hydrolase -
IMY63_RS25765 5253364..5254305 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IMY63_RS25770 5254350..5254787 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IMY63_RS25775 5254784..5255644 - 861 WP_002882807.1 virulence factor BrkB family protein -
IMY63_RS25780 5255638..5256237 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T177615 WP_002882817.1 NZ_CP063147:c5251986-5251603 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T177615 NZ_CP083598:c1117750-1117478 [Macrococcus armenti]
ATGCCTAAGAAAAAGATACTATTTTATTCAGGTGGTTTTGAAGATTATACGTATTGGCAAACTAACGATAAAACTGTATT
AAAGAAAATAAAAAAACTAATAAAGAGTATTATGATTGATGGTGCAAATGAAGGTATAGGTAAGCCAGAAAAATTATTAG
GAAACTATACAGGTTGCTATAGCAGAAGGATCACTCATGAACATAGGCTCGTATATAAGATAGAAGATGATTCAATATGT
ATTTTACAATGTCGTTTTCATTATAAAAGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT177615 WP_004150355.1 NZ_CP063147:c5252228-5251986 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT177615 NZ_CP083598:c1118000-1117743 [Macrococcus armenti]
ATGAAAGTCACAACTTATTCTAATGCAAGATCAAATTTTAGAAAAATAATAGATATTGTTAATGAAGACTGTGAACCTTA
CACAATCACAACAAATACGCATAATGCCGTGATATTATCTGAACAAGATTATAATAGTATGATTGAAACACTTTACTTAC
AGAGTACACCAACGAATGCCGAAAGATTACAAAAAGCGATTGATGAATCAATTGATAAAAATAACTTACTTCAAGTTGAG
ATTGATTTAGATGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References