Detailed information of TA system TA177614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4927553..4929234
• Replicon: chromosome
• Accession: NZ_CP063147
• Organism: Klebsiella pneumoniae strain KP19-3088

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: IMY63_RS24245
• Protein ID: WP_193525093.1
• Coordinates: 4927553..4928470 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: W8UPQ8
• Locus tag: IMY63_RS24250
• Protein ID: WP_004151732.1
• Coordinates: 4928476..4929234 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMY63_RS24225	4922859..4924379	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
IMY63_RS24230	4924376..4925374	+	999	WP_002885172.1	ribose ABC transporter permease	-
IMY63_RS24235	4925405..4926346	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
IMY63_RS24240	4926421..4927428	+	1008	WP_004151731.1	carbohydrate kinase family protein	-
IMY63_RS24245	4927553..4928470	-	918	WP_193525093.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
IMY63_RS24250	4928476..4929234	-	759	WP_004151732.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
IMY63_RS24255	4929320..4930048	-	729	WP_002885152.1	response regulator transcription factor	-
IMY63_RS24260	4930321..4932468	+	2148	WP_077598858.1	formate dehydrogenase subunit alpha	-
IMY63_RS24265	4932698..4933384	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34657.17 Da
• Isoelectric Point: 5.7772

>T177614 WP_193525093.1 NZ_CP063147:c4928470-4927553 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIVVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T177614 NZ_CP083598:1113646-1113906 [Macrococcus armenti]
ATGTACAAAATTGTAATGAAATCTAGTGTTAAGAAAGATTTAAAGAAGATAAAAAACACGCACTTAGAAACGAATTTTCT
AAAAATCATTAATCAGCTTAAAGAAAATCCATATAATAATAACCATTCTTTCGAAAAACTAATCCCCCCAATAAAGGGGT
TTTATTCGAGAAGGATAAATGTACAACATCGTGTTGTTTATACTGTAAATGACGAAGATAAAATTGTAACAATCTATTCA
GCATATGGACATTATGAGTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28564.92 Da
• Isoelectric Point: 10.0870

>AT177614 WP_004151732.1 NZ_CP063147:c4929234-4928476 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLSFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT177614 NZ_CP083598:1113395-1113646 [Macrococcus armenti]
ATGAATACTTATACACCAACAAGTGCTAGAAAAGATTTTTTCAACATCATCAAATCTGTTAATGAAGATAGTAAAGAAAT
ATATATCGCTCCAACTAAAATTGGAGAAAAAGGTGCTGTTTTAGTCGGTGAAGATGATTGGAATGCTATTCAGGAAACTT
TATTCTTATTAGAAAAAGGTATCGCTTATCAAATTGAACAACGTAAAGACGAAGAAGAAATTGATTTTGACAGTACTTGG
GATAATATATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GQB5