Detailed information of TA system TA177597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 619923..620508
• Replicon: chromosome
• Accession: NZ_CP063145
• Organism: Cruoricaptor ignavus strain M1214

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: IMZ16_RS02840
• Protein ID: WP_193440778.1
• Coordinates: 620209..620508 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1M6GJZ2
• Locus tag: IMZ16_RS02835
• Protein ID: WP_073180424.1
• Coordinates: 619923..620207 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMZ16_RS02820	615937..616485	+	549	WP_193440402.1	threonylcarbamoyl-AMP synthase	-
IMZ16_RS02825	616550..618391	-	1842	WP_193440403.1	hypothetical protein	-
IMZ16_RS02830	618497..619921	+	1425	WP_193440404.1	CCA tRNA nucleotidyltransferase	-
IMZ16_RS02835	619923..620207	-	285	WP_073180424.1	putative addiction module antidote protein	Antitoxin
IMZ16_RS02840	620209..620508	-	300	WP_193440778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IMZ16_RS02845	620595..621644	-	1050	WP_193440405.1	hypothetical protein	-
IMZ16_RS02850	621658..623484	-	1827	WP_193440406.1	TonB-dependent receptor	-
IMZ16_RS02855	623883..624173	+	291	WP_193440407.1	DUF2752 domain-containing protein	-
IMZ16_RS02860	624170..625450	-	1281	WP_193440408.1	glutamate-1-semialdehyde 2,1-aminomutase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-92)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11623.68 Da
• Isoelectric Point: 10.6216

>T177597 WP_193440778.1 NZ_CP063145:c620508-620209 [Cruoricaptor ignavus]
MFIIEKTAEFDRWFRKLKDLTARAKILFRIQKLENEGHFGDCKPVGNGISEMRINFAKGYRVYFRETSGKIIILLIGGDK
SSQQKDVEKAKKIWEKIKK

Nucleotide

• Length: 300 bp

>T177597 NZ_CP083585:3581479-3582021 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCACATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTAAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAGCCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTGGGGGTGGCGCG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10143.64 Da
• Isoelectric Point: 4.2220

>AT177597 WP_073180424.1 NZ_CP063145:c620207-619923 [Cruoricaptor ignavus]
MEVSRFDIADYLDSNEMIAEYLNVVLEEGEDSDVVIAIGNVAKAIGMTKIAEETGMSRPSLYKAFSDGAKPQFSTVMKVL
KAVGGQIKVEPITA

Nucleotide

• Length: 285 bp

>AT177597 NZ_CP083585:3581184-3581495 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAGACTTCGTGAAGCTGCGCAAGCCGTTGGGTTAGATTTAAGCGCCTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGCGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCC
GAACCTGCTCAACCGACGCTCGCACTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A1M6GJZ2