Detailed information of TA system TA177588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 702303..702947
• Replicon: chromosome
• Accession: NZ_CP063127
• Organism: Haemophilus influenzae strain M1C112_1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q4QKY0
• Locus tag: INP89_RS03475
• Protein ID: WP_005643265.1
• Coordinates: 702762..702947 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q4QKX9
• Locus tag: INP89_RS03470
• Protein ID: WP_005692602.1
• Coordinates: 702303..702731 (-)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INP89_RS03430	697669..698637	-	969	WP_110426138.1	helix-turn-helix transcriptional regulator	-
INP89_RS03435	698771..698977	+	207	WP_006995067.1	helix-turn-helix domain-containing protein	-
INP89_RS03440	699021..699473	+	453	WP_005651123.1	hypothetical protein	-
INP89_RS03445	699519..699752	+	234	WP_005651121.1	helix-turn-helix domain-containing protein	-
INP89_RS03450	699754..700581	+	828	WP_110426137.1	helix-turn-helix domain-containing protein	-
INP89_RS03455	700566..701201	+	636	WP_011272502.1	replication P	-
INP89_RS03460	701198..701788	+	591	WP_005692598.1	N-6-adenine methyltransferase	-
INP89_RS03465	701793..702302	+	510	WP_005692600.1	DUF1367 family protein	-
INP89_RS03470	702303..702731	-	429	WP_005692602.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
INP89_RS03475	702762..702947	-	186	WP_005643265.1	type II toxin-antitoxin system HicA family toxin	Toxin
INP89_RS03480	703103..703690	+	588	WP_197541689.1	recombination protein NinG	-
INP89_RS03485	703692..704057	+	366	WP_197541690.1	RNA polymerase subunit sigma-70	-
INP89_RS03490	704120..704980	+	861	WP_116938474.1	hypothetical protein	-
INP89_RS03495	704991..706139	+	1149	WP_197541691.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
INP89_RS03500	706300..706485	+	186	WP_005651099.1	hypothetical protein	-
INP89_RS03505	706871..707182	+	312	WP_005651098.1	hypothetical protein	-
INP89_RS03510	707301..707552	+	252	WP_005688811.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	licD	667275..722368	55093

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-134)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6716.88 Da
• Isoelectric Point: 11.1595

>T177588 WP_005643265.1 NZ_CP063127:c702947-702762 [Haemophilus influenzae]
MRSSDLIKELKSAGCTFVRHGKGDHQIWQSPITGKTFPVPHPKQHVPIGTLRSIKKSAGLL

Nucleotide

• Length: 186 bp

>T177588 NZ_CP083585:2279291-2279578 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 16458.45 Da
• Isoelectric Point: 4.3241

>AT177588 WP_005692602.1 NZ_CP063127:c702731-702303 [Haemophilus influenzae]
MLFTIGIETPTNENEAYGITVPALFTEEYSCFSAADTLEEIPTQVTDAIHSILEMMFEDGIDINELQDKGYRHYQTQEDF
NYCDTWLLLDVDISAYQGKRHRINISLPEYLIKRIDSRVASNPIYKDRSHFLAIASQKELRQ

Nucleotide

• Length: 429 bp

>AT177588 NZ_CP083585:2279052-2279291 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: Q4QKY0

Antitoxin

• AlphaFold DB: Q4QKX9