Detailed information of TA system TA177580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3719752..3720367
• Replicon: chromosome
• Accession: NZ_CP063073
• Organism: Pseudomonas poae strain PMA22

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0W0I427
• Locus tag: IMF22_RS16580
• Protein ID: WP_017736377.1
• Coordinates: 3719752..3719934 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IMF22_RS16585
• Protein ID: WP_159954791.1
• Coordinates: 3719957..3720367 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMF22_RS16550	3715171..3715476	+	306	WP_197624100.1	metal ABC transporter ATPase	-
IMF22_RS16555	3715704..3715949	+	246	WP_197624102.1	type II toxin-antitoxin system ParD family antitoxin	-
IMF22_RS16560	3715942..3716232	+	291	WP_197624104.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IMF22_RS16565	3716242..3717435	-	1194	WP_197624106.1	MFS transporter	-
IMF22_RS16570	3717432..3718595	-	1164	WP_197624108.1	MFS transporter	-
IMF22_RS16575	3718691..3719584	+	894	WP_197624110.1	LysR family transcriptional regulator	-
IMF22_RS16580	3719752..3719934	+	183	WP_017736377.1	type II toxin-antitoxin system HicA family toxin	Toxin
IMF22_RS16585	3719957..3720367	+	411	WP_159954791.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IMF22_RS16590	3720457..3720957	+	501	WP_197624112.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
IMF22_RS16595	3721072..3721497	+	426	WP_159954789.1	low affinity iron permease family protein	-
IMF22_RS16600	3721551..3722078	-	528	WP_005785958.1	DUF4142 domain-containing protein	-
IMF22_RS16605	3722198..3722839	-	642	WP_159954788.1	LysE family translocator	-
IMF22_RS16610	3722894..3723304	-	411	WP_197624114.1	fosfomycin resistance glutathione transferase	-
IMF22_RS16615	3723323..3724246	-	924	WP_197624117.1	LysR family transcriptional regulator	-
IMF22_RS16620	3724315..3724803	+	489	WP_197624120.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6791.96 Da
• Isoelectric Point: 10.7532

>T177580 WP_017736377.1 NZ_CP063073:3719752-3719934 [Pseudomonas poae]
VDSRYLIGHIVADGWYLVRTRGSHHHFKHPTKPGLVTIPHPKKDLLDKTAKSILKQALLS

Nucleotide

• Length: 183 bp

>T177580 NZ_CP063073:3719752-3719934 [Pseudomonas poae]
GTGGATAGCCGATATTTGATAGGTCACATCGTTGCGGACGGCTGGTATCTGGTGCGCACCCGAGGCAGTCATCATCACTT
CAAGCACCCGACAAAACCGGGGCTGGTGACGATTCCTCATCCAAAGAAGGACCTGTTGGACAAGACTGCCAAGAGCATTT
TGAAACAGGCCCTGTTGAGCTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14811.72 Da
• Isoelectric Point: 4.3129

>AT177580 WP_159954791.1 NZ_CP063073:3719957-3720367 [Pseudomonas poae]
MLYPIAISTGDEDHAWGVEVPDIPGCYSAGDDLDEAMAMAREAIEGHFEILAEDGAPIPTAQKVTLHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLNHPEEKSRSGFLASAALKVLQQDR

Nucleotide

• Length: 411 bp

>AT177580 NZ_CP063073:3719957-3720367 [Pseudomonas poae]
ATGCTTTACCCCATCGCAATTTCAACCGGTGACGAGGACCACGCCTGGGGCGTGGAAGTCCCGGACATTCCCGGCTGCTA
CTCAGCTGGCGATGACCTGGACGAGGCCATGGCCATGGCCCGGGAGGCCATCGAGGGGCATTTCGAGATCCTCGCGGAAG
ATGGCGCGCCGATTCCCACGGCGCAAAAAGTTACCTTGCACGCAGCGAATCCCCAATACGCCGGCTGCACCTGGGCTGTG
GTGGACATTGATGTCACCAAATACCTGGGCAAAGCGCAGAAGCTCAACATTACCTTGCCGGGTTATCTGTTGAATCGCAT
CGATGAATATGTACTGAACCACCCGGAAGAAAAAAGCCGCTCGGGGTTTCTGGCGTCCGCCGCATTGAAAGTATTGCAAC
AGGACCGATGA

Structures

Toxin

• AlphaFold DB: A0A0W0I427