Detailed information of TA system TA177579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3367726..3368330
• Replicon: chromosome
• Accession: NZ_CP063073
• Organism: Pseudomonas poae strain PMA22

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S2UAI9
• Locus tag: IMF22_RS14820
• Protein ID: WP_065901605.1
• Coordinates: 3368142..3368330 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IMF22_RS14815
• Protein ID: WP_159955072.1
• Coordinates: 3367726..3368130 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMF22_RS14790	3362746..3363618	+	873	WP_197623434.1	urea transporter	-
IMF22_RS14795	3363650..3364474	+	825	WP_197623437.1	ion transporter	-
IMF22_RS14800	3364580..3365584	+	1005	WP_159955075.1	sulfate ABC transporter substrate-binding protein	-
IMF22_RS14805	3365721..3366344	-	624	WP_197623440.1	hypothetical protein	-
IMF22_RS14810	3366523..3367719	+	1197	WP_177015962.1	MFS transporter	-
IMF22_RS14815	3367726..3368130	-	405	WP_159955072.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IMF22_RS14820	3368142..3368330	-	189	WP_065901605.1	type II toxin-antitoxin system HicA family toxin	Toxin
IMF22_RS14825	3368558..3369214	+	657	WP_197623443.1	AhpC/TSA family protein	-
IMF22_RS14830	3369244..3370125	+	882	WP_197623446.1	LysR family transcriptional regulator	-
IMF22_RS14835	3370190..3371032	-	843	WP_197623449.1	M10 family metallopeptidase C-terminal domain-containing protein	-
IMF22_RS14840	3371128..3372204	-	1077	WP_197623452.1	M10 family metallopeptidase C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7219.42 Da
• Isoelectric Point: 11.2000

>T177579 WP_065901605.1 NZ_CP063073:c3368330-3368142 [Pseudomonas poae]
VQSRLLIKELQEAGWVLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVRSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T177579 NZ_CP083585:262877-263152 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGAATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14696.67 Da
• Isoelectric Point: 4.4776

>AT177579 WP_159955072.1 NZ_CP063073:c3368130-3367726 [Pseudomonas poae]
MQYPMCIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGPIPMPTSVANHHANEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT177579 NZ_CP083585:263149-263508 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A1S2UAI9