Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 905261..905890 Replicon chromosome
Accession NZ_CP063071
Organism Listeria seeligeri strain Sr73

Toxin (Protein)


Gene name pemK Uniprot ID A0A6Z2PJ29
Locus tag IMX23_RS04275 Protein ID WP_031695189.1
Coordinates 905543..905890 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID E3ZNL6
Locus tag IMX23_RS04270 Protein ID WP_003746456.1
Coordinates 905261..905539 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMX23_RS04250 900574..902061 + 1488 WP_075702881.1 PH domain-containing protein -
IMX23_RS04255 902194..903573 + 1380 WP_196318949.1 protoporphyrinogen oxidase -
IMX23_RS04260 903575..903931 + 357 WP_196318950.1 holo-ACP synthase -
IMX23_RS04265 903949..905055 + 1107 WP_196318951.1 alanine racemase -
IMX23_RS04270 905261..905539 + 279 WP_003746456.1 CopG family ribbon-helix-helix protein Antitoxin
IMX23_RS04275 905543..905890 + 348 WP_031695189.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IMX23_RS04280 906139..906975 + 837 WP_003751697.1 STAS domain-containing protein -
IMX23_RS04285 906981..907337 + 357 WP_003721457.1 STAS domain-containing protein -
IMX23_RS04290 907340..907750 + 411 WP_046326401.1 anti-sigma regulatory factor -
IMX23_RS04295 907767..908771 + 1005 WP_075702884.1 PP2C family protein-serine/threonine phosphatase -
IMX23_RS04300 908910..909254 + 345 WP_003751701.1 anti sigma b factor antagonist RsbV -
IMX23_RS04305 909238..909711 + 474 WP_003751702.1 anti-sigma B factor RsbW -
IMX23_RS04310 909689..910468 + 780 WP_003746475.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12801.89 Da        Isoelectric Point: 7.2038

>T177576 WP_031695189.1 NZ_CP063071:905543-905890 [Listeria seeligeri]
MMVKRGDVYYADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAKIQKAKLPTHVEATRKDGFERDSVILLEQIR
TIDKQRLTDKITHLDEELMAKVNKALEVSLGVVEF

Download         Length: 348 bp

>T177576 NZ_CP083585:178596-178703 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGTATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10591.11 Da        Isoelectric Point: 4.2980

>AT177576 WP_003746456.1 NZ_CP063071:905261-905539 [Listeria seeligeri]
VLEKEKRMVISVELTQEMVQELDVVVEKEKMGRSEVIMEATQQFLQEKRARELRDEMERGYAEMATINFAIACECTHVEA
EAEDRNISILGG

Download         Length: 279 bp

>AT177576 NZ_CP083585:c178548-178482 [Escherichia coli]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6Z2PJ29

Antitoxin

Source ID Structure
AlphaFold DB A0A7X0X0M3

References