Detailed information of TA system TA177574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2751159..2751760
• Replicon: chromosome
• Accession: NZ_CP063057
• Organism: Comamonas thiooxydans strain ZDHYF418

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: INP81_RS12540
• Protein ID: WP_003055100.1
• Coordinates: 2751159..2751545 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7WTR2
• Locus tag: INP81_RS12545
• Protein ID: WP_003055097.1
• Coordinates: 2751545..2751760 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INP81_RS12515	2746569..2746934	+	366	WP_001277463.1	mercury resistance co-regulator MerD	-
INP81_RS12520	2746931..2747167	+	237	WP_003055146.1	broad-spectrum mercury transporter MerE	-
INP81_RS12525	2747233..2747445	+	213	WP_000993245.1	DUF3330 domain-containing protein	-
INP81_RS12530	2747575..2748135	+	561	WP_003055107.1	recombinase family protein	-
INP81_RS12535	2748138..2751106	+	2969	Protein_2414	Tn3 family transposase	-
INP81_RS12540	2751159..2751545	-	387	WP_003055100.1	type II toxin-antitoxin system VapC family toxin	Toxin
INP81_RS12545	2751545..2751760	-	216	WP_003055097.1	DUF2191 domain-containing protein	Antitoxin
INP81_RS12550	2751846..2752670	+	825	WP_003055094.1	SprT-like domain-containing protein	-
INP81_RS12555	2752985..2755489	-	2505	WP_081007841.1	heavy metal translocating P-type ATPase	-
INP81_RS12560	2755774..2756700	-	927	WP_003071641.1	copper homeostasis membrane protein CopD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• Domain of unknown function DUF2281 (6-73)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13816.18 Da
• Isoelectric Point: 5.9593

>T177574 WP_003055100.1 NZ_CP063057:c2751545-2751159 [Comamonas thiooxydans]
MPVSVLVDTSVWVRHFREADIHLQELLLQDSVLMHPMVHAELACGTPPAPRADTLAALAMLRPSQEASIPETLAFLEKNK
LFGKGCGLVDLSLLASTLITPGALLWTADRRLAELAAQLGVAYAIPTH

Nucleotide

• Length: 387 bp

>T177574 NZ_CP083582:c7225009-7224614 [Kovacikia minuta CCNUW1]
ATGAGTGCAGTTTTACTAGACACCCATGCTTTTATTTGGCTTGTAGAAGATAGCCCCAAGCTTCCACAGCCTATCAAAGA
AAGAATAGCTGTGACGAACGATATTTTTGTCAGCATTGCCAGTTTTTGGGAAATTGGAATAAAGCTTAAAAAAGCGCAGA
TTTTTTTGAGCTGTGATTTCAACGAAATTGAGTCCAGATTTCTGGAAACCGACTTACAGTTACTCCCAATCTCGATACAA
GATACGATTCAACAATATAATCTTCCCTTTTATTTAGCAGAGCACAAAGATCCCTTTGATCGTATTATTATTTCTCAGGC
AATCAGTCGATCTCTCCCTCTTGTGAGTAAAGACAAAGAATTTGATGCTTATCCCATCGAAAAGGTATGGGCATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7448.46 Da
• Isoelectric Point: 6.2846

>AT177574 WP_003055097.1 NZ_CP063057:c2751760-2751545 [Comamonas thiooxydans]
MRSTFTIDDEVVNRARAVAAPGIAVPELVRLALETFTRVEAGKRLAALGGSAPDMPDVPRRGSAAEAEGSR

Nucleotide

• Length: 216 bp

>AT177574 NZ_CP083582:c7225233-7225009 [Kovacikia minuta CCNUW1]
ATGATAAAACCAACTATTCTAGAAAAGCTGGAAAAGCTGCCTGAAGCACTTCAAACAGAAGTACTTCATTACATTGAATT
TCTGCTAGAAAAACATACGACACCACCTATCCAAAAAGAAACACCCAAAAAGCGTCGTTTGGCTGGTTCTATGAAAGGCA
CATTTGTGTTGCCGTTACCAGATAACTTTGATGAACCTCTAGAAGAATTTGAGGACTATATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A4Y8TDR8