Detailed information of TA system TA177573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2649578..2651114
• Replicon: chromosome
• Accession: NZ_CP063057
• Organism: Comamonas thiooxydans strain ZDHYF418

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: INP81_RS12075
• Protein ID: WP_012838314.1
• Coordinates: 2649816..2651114 (+)
• Length: 433 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: INP81_RS12070
• Protein ID: WP_012838313.1
• Coordinates: 2649578..2649826 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INP81_RS12055	2646277..2647152	+	876	WP_054074233.1	hypothetical protein	-
INP81_RS12060	2647245..2649083	+	1839	WP_054074234.1	dihydrolipoyl dehydrogenase	-
INP81_RS12065	2649248..2649406	+	159	WP_041744108.1	hypothetical protein	-
INP81_RS12070	2649578..2649826	+	249	WP_012838313.1	helix-turn-helix transcriptional regulator	Antitoxin
INP81_RS12075	2649816..2651114	+	1299	WP_012838314.1	type II toxin-antitoxin system HipA family toxin	Toxin
INP81_RS12080	2651348..2651824	+	477	WP_080692925.1	GFA family protein	-
INP81_RS12085	2651957..2653327	-	1371	WP_012838316.1	MFS transporter	-
INP81_RS12090	2654139..2654495	-	357	WP_054074235.1	alkylphosphonate utilization protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-60)

Sequences

Toxin

Protein

• Length: 433 a.a.
• Molecular weight: 47029.09 Da
• Isoelectric Point: 7.2921

>T177573 WP_012838314.1 NZ_CP063057:2649816-2651114 [Comamonas thiooxydans]
MTHELEVWLFDERVGSLSLVHGRLSFAYATDWLALPQATALSQSLPLQAKPFDDHQTRPFFAGLLPEGQMRRLIAQQFQV
SGQNDFALLDHIGGECAGAVTFLEPGQALPQTNGAGATGAPGDVEWLSDQKLLALLDELPRRPMLAGDDGLRLSLAGAQD
KLPVVVDESIPGAVRIGLPRNGAPSSHILKPAIHAVEDSVANEGFCMLLAQVMGLRPANARIQSVLGRSFLLVKRYDREL
NAQDLHQPQLQRLHQEDFCQALGVVPELKYQNEGGPGLAQCFALLRSVTRPSAPQVLRLLDGAIFNALIGNHDAHGKNFS
LLYGGRAPVLAPFYDLLATAIYPQLTPKMAMKIGSKYKFSELEARHWEQFAEEAGLAKAATRKRLQQLANELPAAARKLQ
AAPPHGFAGNAVVTQIVQLIEQRCALTLRRLA

Nucleotide

• Length: 1299 bp

>T177573 NZ_CP083582:c4379764-4379387 [Kovacikia minuta CCNUW1]
ATGCTGCTAGATACTCATACATTGCTCTGGTTTCTCAATAATGAATTCAAGTTGCCAGCCTCAATCAAACAACAGATAGA
AAATACTGAATTTGTCTTCGCTAGTATTGCTTCAATTTGGGAAATCGCTATCAAAGTTAATGTTGGAAAGTTGACACTTC
TGAGTCCGTTAGAGACCATTCAGCTAAACATGGTTGCCTTGAGTATTCAAGAATTAACGATCTCGTTTGCTGATGTGCAA
TGCTACATTAACTTGCCACTTCACCATCGTGATCCATTTGATCGCATGTTAATTGCTCAGGCAATCAACCATTCCCTTGT
GATTGTAAGTGCCGATACCGCATTTGACGCTTATCCCATTCAACGGGTATGGGCATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8529.72 Da
• Isoelectric Point: 5.6051

>AT177573 WP_012838313.1 NZ_CP063057:2649578-2649826 [Comamonas thiooxydans]
MSTIQTTEQLGAALRTARKQLGLTQPQLALAAGVGTRFIVDLEAGKPTVRLEHVLRVIDALGGELQLGGLPNTASEGADH
DA

Nucleotide

• Length: 249 bp

>AT177573 NZ_CP083582:c4379991-4379773 [Kovacikia minuta CCNUW1]
ATGATACAACCTGCCATTCTCAAAAAACTGGAGAGCCTTCCTGAACCTCTACAATTAAAAGTGCTTCATTACATTGATTC
TTTAATTGAGGAGCAGGATAAAACTTCAAAGCAAGAAAATGCTCCTAAAAAATATCGTGTTGCAGGAACAATGAAAGGCA
TGATTATCATGTCTGATGATTTTGATGAACCACTTGAAGATCTAAAAGACTATATGTAA