Detailed information of TA system TA177572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1801109..1802148
• Replicon: chromosome
• Accession: NZ_CP063057
• Organism: Comamonas thiooxydans strain ZDHYF418

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: INP81_RS08235
• Protein ID: WP_177341014.1
• Coordinates: 1801109..1801684 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: INP81_RS08240
• Protein ID: WP_177341015.1
• Coordinates: 1801684..1802148 (-)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INP81_RS08225	1799371..1800441	-	1071	WP_197577658.1	IS110 family transposase	-
INP81_RS08230	1800614..1800940	+	327	WP_177341136.1	helix-turn-helix domain-containing protein	-
INP81_RS08235	1801109..1801684	-	576	WP_177341014.1	PIN domain-containing protein	Toxin
INP81_RS08240	1801684..1802148	-	465	WP_177341015.1	helix-turn-helix domain-containing protein	Antitoxin
INP81_RS08245	1802699..1804033	-	1335	WP_177341137.1	hypothetical protein	-
INP81_RS08250	1804082..1804963	-	882	WP_177341016.1	ParB N-terminal domain-containing protein	-
INP81_RS08255	1804960..1805862	-	903	WP_177341017.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1737554..1851701	114147

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21393.69 Da
• Isoelectric Point: 5.6378

>T177572 WP_177341014.1 NZ_CP063057:c1801684-1801109 [Comamonas thiooxydans]
MRSSPFTVVYDACVMYPAPLRSFLMYVSLEGCYRARWTAQIHDEWKRNLLKNRPDFTQEKLDKISSLMGQAVPDCLVTGY
DALINGLTLPDQDDRHVLAAAIRCGAGVIVTMNLKDLPPAALSQYGVEAQHPDEFLFDLMDLDLAAVLNAASKHRASLKN
PELTAQEYLDMLERQGLTQTCRKLAAYSLLI

Nucleotide

• Length: 576 bp

>T177572 NZ_CP083582:c586759-586472 [Kovacikia minuta CCNUW1]
ATGAGGATCATCTGGACACAGCAGGCGATCTCAGATCTTAACCACGCTTACGATTACATCGCTGAAACTGCTCCCGAATC
TGGCACCGCTATCATTGAACGAATAGAGAAATCGCTAGAAGCTTTAAGCAACTTCCCAGAAATGGGACGAAAGGGACGCG
TCAACAACACACGGGAACTGATAGTACCGAAAACCCCATTCATTTTGCCCTATCGAATTCGCAAAAATCGCATCGAAATT
TTAGGCGTGATTCACGCCACCCGACGCTGGCCCAGCAGTTTTGAGTAA

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 16781.08 Da
• Isoelectric Point: 5.5804

>AT177572 WP_177341015.1 NZ_CP063057:c1802148-1801684 [Comamonas thiooxydans]
MNAIANDKVMLPHATEKQAATEGQRALAAYLSTKAETQLIQITSTDGQQHPIELPTSALRLLQEVLAELALGNAVKVVPV
HAELTTQEAADLLNVSRPYVVQLMESGELPFHKAGTHRRVKFSDLQAYKEKRDQSSAEAMTALSALDQELGMGY

Nucleotide

• Length: 465 bp

>AT177572 NZ_CP083582:c586992-586756 [Kovacikia minuta CCNUW1]
ATGAGCAAGGAAACGATTACTTTTCGACTTGATCAAGAGAAGCGGGCAGCGCTAGATGAAATTGCGGAGGGGATGGATCG
CGATCGCTCCTACATCTTGAACGAAGCGATTGATCTCTATCTAGAAGTTCACCACTGGCAGGTAGAACATATTTTGGCAG
GGATGCAACAGGCGGATGCTGAGGAGTTTGCCACGGATGCTGAAGTAGCAGCGGCATTTGCTCGCTGGCGTCAATGA