Detailed information of TA system TA177563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2684178..2685398
• Replicon: chromosome
• Accession: NZ_CP063047
• Organism: Pseudomonas aeruginosa strain KC-Tt-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: V6AG16
• Locus tag: IL983_RS12155
• Protein ID: WP_003088107.1
• Coordinates: 2684820..2685398 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IL983_RS12150
• Protein ID: WP_003132728.1
• Coordinates: 2684178..2684732 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IL983_RS12125	2679746..2680297	+	552	WP_034036786.1	helix-turn-helix transcriptional regulator	-
IL983_RS12130	2680294..2680692	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
IL983_RS12135	2680705..2681028	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
IL983_RS12140	2681359..2681820	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
IL983_RS12145	2681825..2684020	+	2196	WP_034036785.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
IL983_RS12150	2684178..2684732	+	555	WP_003132728.1	helix-turn-helix domain-containing protein	Antitoxin
IL983_RS12155	2684820..2685398	+	579	WP_003088107.1	HD domain-containing protein	Toxin
IL983_RS12160	2685507..2686694	-	1188	WP_003088106.1	HlyD family efflux transporter periplasmic adaptor subunit	-
IL983_RS12165	2686684..2688855	-	2172	WP_003088105.1	type I secretion system permease/ATPase	-
IL983_RS12170	2688845..2690122	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-187)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21885.74 Da
• Isoelectric Point: 5.3655

>T177563 WP_003088107.1 NZ_CP063047:2684820-2685398 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDAKTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T177563 NZ_CP083532:23619-23900 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCTACCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20470.63 Da
• Isoelectric Point: 8.1590

>AT177563 WP_003132728.1 NZ_CP063047:2684178-2684732 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLESAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT177563 NZ_CP083532:23378-23629 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCTACCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: V6AG16