Detailed information of TA system TA177553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4971033..4972621
• Replicon: chromosome
• Accession: NZ_CP063046
• Organism: Escherichia coli strain KC-Dl-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: IL980_RS23905
• Protein ID: WP_001125475.1
• Coordinates: 4971033..4972355 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: IL980_RS23910
• Protein ID: WP_001332145.1
• Coordinates: 4972355..4972621 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IL980_RS23865	4966672..4966905	-	234	WP_000473544.1	hypothetical protein	-
IL980_RS23870	4966940..4967191	-	252	WP_001536107.1	hypothetical protein	-
IL980_RS23875	4967194..4967511	-	318	WP_000073835.1	hypothetical protein	-
IL980_RS23880	4967523..4968575	-	1053	WP_000568107.1	hypothetical protein	-
IL980_RS23885	4968747..4969193	+	447	WP_000527682.1	helix-turn-helix domain-containing protein	-
IL980_RS23890	4969293..4969715	+	423	WP_001527022.1	hypothetical protein	-
IL980_RS23895	4969783..4970577	+	795	WP_001527023.1	hypothetical protein	-
IL980_RS23900	4970588..4970854	+	267	WP_001527024.1	hypothetical protein	-
IL980_RS23905	4971033..4972355	-	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
IL980_RS23910	4972355..4972621	-	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
IL980_RS23915	4972929..4973687	+	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
IL980_RS23920	4973691..4974605	-	915	WP_001306069.1	bestrophin family protein	-
IL980_RS23925	4974802..4976253	-	1452	WP_000854636.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T177553 WP_001125475.1 NZ_CP063046:c4972355-4971033 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T177553 NZ_CP083530:c4196145-4195951 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT177553 WP_001332145.1 NZ_CP063046:c4972621-4972355 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT177553 NZ_CP083530:4195774-4195952 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGAAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8