Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5173058..5173683 Replicon chromosome
Accession NZ_CP063023
Organism Klebsiella pneumoniae strain CriePir45

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GJJ13_RS25005 Protein ID WP_002882817.1
Coordinates 5173058..5173441 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GJJ13_RS25010 Protein ID WP_004150355.1
Coordinates 5173441..5173683 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJJ13_RS24990 5170424..5171326 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GJJ13_RS24995 5171323..5171958 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GJJ13_RS25000 5171955..5172884 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GJJ13_RS25005 5173058..5173441 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GJJ13_RS25010 5173441..5173683 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GJJ13_RS25015 5173888..5174805 + 918 WP_015959199.1 alpha/beta hydrolase -
GJJ13_RS25020 5174819..5175760 - 942 WP_153932582.1 fatty acid biosynthesis protein FabY -
GJJ13_RS25025 5175805..5176242 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GJJ13_RS25030 5176239..5177099 - 861 WP_004146232.1 virulence factor BrkB family protein -
GJJ13_RS25035 5177093..5177692 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T177534 WP_002882817.1 NZ_CP063023:c5173441-5173058 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T177534 NZ_CP083514:c23053-22778 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTACGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT177534 WP_004150355.1 NZ_CP063023:c5173683-5173441 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT177534 NZ_CP083514:c23337-23053 [Escherichia coli]
ATGCAAATGAAAAACAATAGTGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References