Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5418923..5419548 Replicon chromosome
Accession NZ_CP063019
Organism Klebsiella pneumoniae strain CriePir75

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GJJ10_RS26745 Protein ID WP_002882817.1
Coordinates 5418923..5419306 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GJJ10_RS26750 Protein ID WP_004150355.1
Coordinates 5419306..5419548 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJJ10_RS26730 5416289..5417191 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GJJ10_RS26735 5417188..5417823 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GJJ10_RS26740 5417820..5418749 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GJJ10_RS26745 5418923..5419306 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GJJ10_RS26750 5419306..5419548 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GJJ10_RS26755 5419753..5420670 + 918 WP_004187175.1 alpha/beta hydrolase -
GJJ10_RS26760 5420684..5421625 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GJJ10_RS26765 5421670..5422107 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GJJ10_RS26770 5422104..5422964 - 861 WP_019725422.1 virulence factor BrkB family protein -
GJJ10_RS26775 5422958..5423557 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T177517 WP_002882817.1 NZ_CP063019:c5419306-5418923 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T177517 NZ_CP083512:c1778290-1777955 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAAAGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT177517 WP_004150355.1 NZ_CP063019:c5419548-5419306 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT177517 NZ_CP083512:c1778538-1778290 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCAGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References