Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5333365..5333990 Replicon chromosome
Accession NZ_CP063011
Organism Klebsiella pneumoniae strain CriePir116

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GJI97_RS26175 Protein ID WP_002882817.1
Coordinates 5333365..5333748 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GJI97_RS26180 Protein ID WP_004150355.1
Coordinates 5333748..5333990 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJI97_RS26160 5330731..5331633 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GJI97_RS26165 5331630..5332265 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GJI97_RS26170 5332262..5333191 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GJI97_RS26175 5333365..5333748 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GJI97_RS26180 5333748..5333990 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GJI97_RS26185 5334195..5335112 + 918 WP_002882812.1 alpha/beta hydrolase -
GJI97_RS26190 5335126..5336067 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GJI97_RS26195 5336112..5336549 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GJI97_RS26200 5336546..5337406 - 861 WP_002882807.1 virulence factor BrkB family protein -
GJI97_RS26205 5337400..5337999 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T177498 WP_002882817.1 NZ_CP063011:c5333748-5333365 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T177498 NZ_CP083503:c2571980-2571684 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT177498 WP_004150355.1 NZ_CP063011:c5333990-5333748 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT177498 NZ_CP083503:c2571682-2571287 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References