Detailed information of TA system TA177497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5009315..5010996
• Replicon: chromosome
• Accession: NZ_CP063011
• Organism: Klebsiella pneumoniae strain CriePir116

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: W8V1Q6
• Locus tag: GJI97_RS24670
• Protein ID: WP_002885155.1
• Coordinates: 5009315..5010232 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: W8UPQ8
• Locus tag: GJI97_RS24675
• Protein ID: WP_004151732.1
• Coordinates: 5010238..5010996 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJI97_RS24650	5004621..5006141	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
GJI97_RS24655	5006138..5007136	+	999	WP_002885172.1	ribose ABC transporter permease	-
GJI97_RS24660	5007167..5008108	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
GJI97_RS24665	5008183..5009190	+	1008	WP_004151731.1	carbohydrate kinase family protein	-
GJI97_RS24670	5009315..5010232	-	918	WP_002885155.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GJI97_RS24675	5010238..5010996	-	759	WP_004151732.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GJI97_RS24680	5011082..5011810	-	729	WP_002885152.1	response regulator transcription factor	-
GJI97_RS24685	5012083..5014230	+	2148	WP_077598858.1	formate dehydrogenase subunit alpha	-
GJI97_RS24690	5014460..5015146	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34687.15 Da
• Isoelectric Point: 5.4707

>T177497 WP_002885155.1 NZ_CP063011:c5010232-5009315 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T177497 NZ_CP083503:2490222-2490599 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGCCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATACGGTGAACTTTCTCGTTGAAAAATACGCACTGATACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAACATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28564.92 Da
• Isoelectric Point: 10.0870

>AT177497 WP_004151732.1 NZ_CP063011:c5010996-5010238 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLSFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT177497 NZ_CP083503:2489764-2490132 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GH11

Antitoxin

• AlphaFold DB: A0A0H3GQB5