Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 20227..20870 Replicon plasmid unnamed1
Accession NZ_CP063004
Organism Klebsiella pneumoniae strain CriePir154

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag GJI94_RS26140 Protein ID WP_000754566.1
Coordinates 20227..20643 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag GJI94_RS26145 Protein ID WP_001261276.1
Coordinates 20640..20870 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJI94_RS26115 17262..17597 + 336 Protein_14 Tn3 family transposase -
GJI94_RS26120 17815..18231 - 417 Protein_15 EstP -
GJI94_RS26125 18240..18686 - 447 Protein_16 restriction endonuclease -
GJI94_RS26130 18685..18885 - 201 Protein_17 IS110 family transposase -
GJI94_RS26135 19167..20023 - 857 Protein_18 IS3-like element ISEc15 family transposase -
GJI94_RS26140 20227..20643 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
GJI94_RS26145 20640..20870 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GJI94_RS26150 21444..21794 + 351 WP_000493378.1 hypothetical protein -
GJI94_RS26155 21845..22588 + 744 WP_000129823.1 hypothetical protein -
GJI94_RS26160 22585..23361 + 777 WP_000015958.1 site-specific integrase -
GJI94_RS26165 23419..23676 - 258 WP_000764642.1 hypothetical protein -
GJI94_RS26170 23805..23909 - 105 WP_032409716.1 hypothetical protein -
GJI94_RS26175 24439..25305 + 867 WP_004118283.1 replication initiation protein -
GJI94_RS26180 25482..25751 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..29625 29625


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T177462 WP_000754566.1 NZ_CP063004:c20643-20227 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T177462 NZ_CP083497:1719827-1720045 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT177462 WP_001261276.1 NZ_CP063004:c20870-20640 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT177462 NZ_CP083497:1719427-1719801 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References