Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5223082..5223707 Replicon chromosome
Accession NZ_CP063003
Organism Klebsiella pneumoniae strain CriePir154

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GJI94_RS25680 Protein ID WP_002882817.1
Coordinates 5223082..5223465 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GJI94_RS25685 Protein ID WP_004150355.1
Coordinates 5223465..5223707 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJI94_RS25665 5220448..5221350 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GJI94_RS25670 5221347..5221982 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GJI94_RS25675 5221979..5222908 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GJI94_RS25680 5223082..5223465 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GJI94_RS25685 5223465..5223707 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GJI94_RS25690 5223912..5224829 + 918 WP_002882812.1 alpha/beta hydrolase -
GJI94_RS25695 5224843..5225784 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GJI94_RS25700 5225829..5226266 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GJI94_RS25705 5226263..5227123 - 861 WP_002882807.1 virulence factor BrkB family protein -
GJI94_RS25710 5227117..5227716 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T177461 WP_002882817.1 NZ_CP063003:c5223465-5223082 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T177461 NZ_CP083497:964598-964705 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT177461 WP_004150355.1 NZ_CP063003:c5223707-5223465 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT177461 NZ_CP083497:c964548-964485 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References