Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17512..18155 Replicon plasmid unnamed1
Accession NZ_CP062998
Organism Klebsiella pneumoniae strain CriePir197

Toxin (Protein)


Gene name vagD Uniprot ID A0A3Q8D6L7
Locus tag GJJ12_RS26055 Protein ID WP_020804312.1
Coordinates 17739..18155 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag GJJ12_RS26050 Protein ID WP_001261276.1
Coordinates 17512..17742 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJJ12_RS26010 13052..13165 + 114 WP_014343462.1 hypothetical protein -
GJJ12_RS26015 13294..13551 + 258 WP_009310077.1 hypothetical protein -
GJJ12_RS26020 13609..14388 - 780 WP_023287113.1 site-specific integrase -
GJJ12_RS26025 14586..15602 - 1017 WP_009309921.1 hypothetical protein -
GJJ12_RS26030 15636..15971 - 336 WP_009309920.1 hypothetical protein -
GJJ12_RS26035 16021..16161 - 141 WP_162898808.1 hypothetical protein -
GJJ12_RS26040 16390..16695 - 306 WP_009309918.1 type II toxin-antitoxin system toxin CcdB -
GJJ12_RS26045 16697..16915 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
GJJ12_RS26050 17512..17742 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GJJ12_RS26055 17739..18155 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin Toxin
GJJ12_RS26060 18196..19074 - 879 WP_009309916.1 restriction endonuclease -
GJJ12_RS26065 19553..20578 + 1026 WP_001101446.1 IS110 family transposase -
GJJ12_RS26070 20897..21865 - 969 WP_077254959.1 IS5 family transposase -
GJJ12_RS26075 21927..22529 - 603 WP_015060010.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkF / mrkJ 1..114497 114497


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15154.59 Da        Isoelectric Point: 7.8637

>T177442 WP_020804312.1 NZ_CP062998:17739-18155 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T177442 NZ_CP083492:3838220-3838327 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT177442 WP_001261276.1 NZ_CP062998:17512-17742 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT177442 NZ_CP083492:c3838172-3838106 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References